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Ozdemir, Filiz, Sever, Arda, Öğünç Keçeci, Yüksel, Incesu, Zerrin. (1399). Resveratrol increases the sensitivity of breast cancer MDA-MB-231 cell line to cisplatin by regulating intrinsic apoptosis. , 24(1), 66-72. doi: 10.22038/ijbms.2020.50485.11501
Filiz Ozdemir; Arda Sever; Yüksel Öğünç Keçeci; Zerrin Incesu. "Resveratrol increases the sensitivity of breast cancer MDA-MB-231 cell line to cisplatin by regulating intrinsic apoptosis". , 24, 1, 1399, 66-72. doi: 10.22038/ijbms.2020.50485.11501
Ozdemir, Filiz, Sever, Arda, Öğünç Keçeci, Yüksel, Incesu, Zerrin. (1399). 'Resveratrol increases the sensitivity of breast cancer MDA-MB-231 cell line to cisplatin by regulating intrinsic apoptosis', , 24(1), pp. 66-72. doi: 10.22038/ijbms.2020.50485.11501
Ozdemir, Filiz, Sever, Arda, Öğünç Keçeci, Yüksel, Incesu, Zerrin. Resveratrol increases the sensitivity of breast cancer MDA-MB-231 cell line to cisplatin by regulating intrinsic apoptosis. , 1399; 24(1): 66-72. doi: 10.22038/ijbms.2020.50485.11501

Resveratrol increases the sensitivity of breast cancer MDA-MB-231 cell line to cisplatin by regulating intrinsic apoptosis

Iranian Journal of Basic Medical Sciences
مقاله 10، دوره 24، شماره 1، فروردین 2021، صفحه 66-72    اصل مقاله (979.69 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.50485.11501
نویسندگان
Filiz Ozdemir* ؛ Arda Sever؛ Yüksel Öğünç Keçeci؛ Zerrin Incesu
Department of Biochemistry, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey
چکیده
Objective(s): Breast cancer is one of the most common types of cancer. Chemotherapeutic agents used during treatment induce cytotoxic effects also on normal cells in the tissues. Anti-oxidants used in combination with chemotherapeutic agents have been shown to reduce toxicity on normal cells to a minimum, and some anti-oxidant substances have chemotherapeutic effects. Cisplatin (CDDP) is a platinum class drug that is used clinically in the treatment of many cancers. Resveratrol (RSV) is a natural polyphenol with potent anti-oxidant and anticancer properties. In this study, we aimed to investigate apoptotic effects of using cisplatin and RSV alone or in combined treatment of MDA-MB-231 cells.
Materials and Methods: The cytotoxic effects of the drugs on MDA-MB-231 cells were determined by MTT method. Subsequently, the change in CDDP-induced apoptotic effect after RSV addition was examined using the AnnexinV FITC labeling, and TUNEL staining method. Activation of caspase-9, -3 in MDA-MB-231 cells was measured by flow cytometer. The mitochondrial membrane potential (MMP), the major factor on the intrinsic pathway, was measured using flowcytometry.
Results: The combined dose (23 μM CDDP + 72 μM RSV) produced more cytotoxicity than the agents used alone, leading to early apoptosis (8.2%), 31% depolarization, and 23% DNA fragmentation. Caspase-9 was found to be 30.5% in this combined group and caspase-3 was 26.3%.
Conclusion: RSV, an effective anti-oxidant, and CDDP as an effective drug in cancer treatment, were found to increase apoptosis when given in the MDA-MB-231 cell.
کلیدواژه‌ها
Apoptosis؛ Breast cancer؛ Cisplatin؛ Combined therapy؛ Resveratrol
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