Yarahmadi, Amir, Moradi Sarabi, Mostafa, Sayahi, Ahmad, Zal, Fatemeh. (1400). Protective effects of quercetin against hyperglycemia-induced oxidative stress in hepatic HepG2 cell line. , 11(3), 269-280. doi: 10.22038/ajp.2020.16913
Amir Yarahmadi; Mostafa Moradi Sarabi; Ahmad Sayahi; Fatemeh Zal. "Protective effects of quercetin against hyperglycemia-induced oxidative stress in hepatic HepG2 cell line". , 11, 3, 1400, 269-280. doi: 10.22038/ajp.2020.16913
Yarahmadi, Amir, Moradi Sarabi, Mostafa, Sayahi, Ahmad, Zal, Fatemeh. (1400). 'Protective effects of quercetin against hyperglycemia-induced oxidative stress in hepatic HepG2 cell line', , 11(3), pp. 269-280. doi: 10.22038/ajp.2020.16913
Yarahmadi, Amir, Moradi Sarabi, Mostafa, Sayahi, Ahmad, Zal, Fatemeh. Protective effects of quercetin against hyperglycemia-induced oxidative stress in hepatic HepG2 cell line. , 1400; 11(3): 269-280. doi: 10.22038/ajp.2020.16913

Protective effects of quercetin against hyperglycemia-induced oxidative stress in hepatic HepG2 cell line

Avicenna Journal of Phytomedicine
مقاله 7، دوره 11، شماره 3، مرداد و شهریور 2021، صفحه 269-280    اصل مقاله (1.11 M)
نوع مقاله: Original Research Article
شناسه دیجیتال (DOI): 10.22038/ajp.2020.16913
نویسندگان
Amir Yarahmadi1؛ Mostafa Moradi Sarabi2؛ Ahmad Sayahi2؛ Fatemeh Zal* 1
1Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
2Department of Biochemistry and Genetics, Lorestan University of Medical Sciences, Faculty of Medicine, Khorramabad, Iran.
چکیده
Objective: Hyperglycemia is a severe consequence of diabetes mellitus (DM). Throughinduction of oxidative stress, it plays a major role in the pathogenesis of several complications in DM. Therefore, new strategies and antioxidants should be implemented inthe treatment of DM. Quercetin is a flavonoid with strong antioxidant capacity found dominantly in vegetables, fruits, leaves, and grains. The current study aimed to investigate quercetin protective effects under D-glucose-induced oxidative stress by assessing antioxidant defense enzymes inHepG2 cells as an in vitro model.
Materials and Methods: HepG2 cells were cultured with different concentrations of D-glucose (5.5, 30 and 50 mM) and/or 25 μM quercetin for 48 and 72 hr, respectively. The effect of treatments on cellular integrity, antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) activity, andcellular levels of glutathione (GSH) and malondialdehyde (MDA) wasdetermined.
Results: D-glucose had various effects on intracellular antioxidant defense atdifferent doses and time-points and quercetin could attenuate oxidative stress and modulate antioxidant defenses.
Conclusion: The results of this study indicated that flavonoid quercetin could be proposed as an agent protecting hepatic HepG2 cells against oxidative stress associated with hyperglycemia.
کلیدواژه‌ها
Flavonoid؛ Quercetin؛ Antioxidant enzymes؛ Hyperglycemia؛ Oxidative stress؛ HepG2 cells
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