Cytotoxic effects of hydroalcoholic extract of Cuscuta chinensis on PC3 and MCF7 cancer cell lines | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 6، دوره 11، شماره 3، مرداد و شهریور 2021، صفحه 258-268 اصل مقاله (949.24 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2020.16902 | ||
| نویسندگان | ||
| Fatemeh Karimi Dermani1؛ Massoud Saidijam2؛ Rezvan Najafi1؛ Shirin Moradkhani3؛ Zahra Mohammadzaheri4؛ Negar Beiranvand5؛ Samane Mohammadi3؛ Noushin Shabab6؛ Ramazan Kalvandi7؛ Fatemeh Zeraati* 5 | ||
| 1Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan Iran | ||
| 2Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 3Department of pharmacognosy, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 4Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan,Iran | ||
| 5Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 6Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran . | ||
| 7Natural Resources Department, Hamadan Agricultural and Natural Resources Research and Education Center, AREEO, Hamadan, Iran | ||
| چکیده | ||
| Objective: Chemoprevention of cancer by application of natural phytochemical compounds has been used to prevent, delay or suppress cancer progression. Cuscuta chinensis a traditional Iranian medicinal herb, has biological properties including anticancer, anti-aging, immuno-stimulatory and antioxidant effects. In this study, anti-proliferative effects of hydroalcoholic extract of C. chinensis on prostate (PC3) and breast (MCF7) cancer cell lines were investigated. Materials and Methods: In the current study, we investigated treatment of PC3 cells with different concentrations of C. chinensis (0, 100, 200, 300, 400, and 500 µg/ml) for 24 and 48 hr; also, MCF7 cells were treated with various concentrations (0-600 µg/ml) of C. chinensis for 48 and 72 hr and cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. mRNA expression of BCL2 Associated X (Bax), B-cell lymphoma 2 (Bcl2), Cysteine-aspartic proteases (Caspase3) and Phosphatase and tensin homolog (PTEN) were analyzed by quantitative real-time PCR. Annexin V/PI staining and lactate dehydrogenase (LDH) cytotoxicity assay were used to detect apoptosis. Results: C. chinensis decreased PC3 and MCF7 cells viability in a dose- and time-dependent manner (p BAX/Bcl2 ratio, Caspase3 and PTEN increased in C. chinensis-treated cells compared to the control group. C. chinensis induced apoptosis (p <0.001) and LDH activity (p Conclusion: Our findings suggest that C. chinensis extract is able to inhibit proliferation and induce apoptosis in PC3 and MCF7 cell lines. Therefore, C. chinensis extract exerts antitumor activity against cancer cells. | ||
| کلیدواژهها | ||
| Cuscuta. chinensis؛ Chemoprevention؛ Prostate cancer؛ Breast cancer؛ Apoptosis | ||
| مراجع | ||
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