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Tazik, Zahra, Rahnama, Kamran, White, James, Soltanloo, Hassan, Hasanpour, Maede, Iranshahi, Mehrdad. (1399). LC-MS based identification of stylosin and tschimgine from fungal endophytes associated with Ferula ovina. , 23(12), 1565-1570. doi: 10.22038/ijbms.2020.46334.10703
Zahra Tazik; Kamran Rahnama; James Francis White; Hassan Soltanloo; Maede Hasanpour; Mehrdad Iranshahi. "LC-MS based identification of stylosin and tschimgine from fungal endophytes associated with Ferula ovina". , 23, 12, 1399, 1565-1570. doi: 10.22038/ijbms.2020.46334.10703
Tazik, Zahra, Rahnama, Kamran, White, James, Soltanloo, Hassan, Hasanpour, Maede, Iranshahi, Mehrdad. (1399). 'LC-MS based identification of stylosin and tschimgine from fungal endophytes associated with Ferula ovina', , 23(12), pp. 1565-1570. doi: 10.22038/ijbms.2020.46334.10703
Tazik, Zahra, Rahnama, Kamran, White, James, Soltanloo, Hassan, Hasanpour, Maede, Iranshahi, Mehrdad. LC-MS based identification of stylosin and tschimgine from fungal endophytes associated with Ferula ovina. , 1399; 23(12): 1565-1570. doi: 10.22038/ijbms.2020.46334.10703

LC-MS based identification of stylosin and tschimgine from fungal endophytes associated with Ferula ovina

Iranian Journal of Basic Medical Sciences
مقاله 8، دوره 23، شماره 12، اسفند 2020، صفحه 1565-1570    اصل مقاله (1 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.46334.10703
نویسندگان
Zahra Tazik1؛ Kamran Rahnama1؛ James Francis White2؛ Hassan Soltanloo3؛ Maede Hasanpour4؛ Mehrdad Iranshahi* 4
1Department of Plant Protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2Department of Biology, Rutgers University, New Brunswick, New Jersey, U.S.A.
3Department of Biotechnology & Plant Breeding, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
4Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Ferula ovina is an Iranian medicinal plant. Tschimgine and stylosin are two of its major monoterpene derivatives. In this study, we proceeded to investigate some fungal endophytes from F. ovina that can produce plant secondary metabolites.
Materials and Methods: The isolated endophytic fungi were fermented in potato dextrose broth (PDB) medium and their extracts were screened for the presence of the plant compounds by liquid chromatography-tandem mass spectrometry (LC-MS). Endophytes identification was performed by morphological and molecular methods. Three markers (ITS, LSU, and TEF1) were used for accurate molecular identification.
Results: Forty isolates from 9 different genera of endophytic fungi were identified, of which two recently reported species of O. ferulica and Pithoascus persicus were able to produce tschimgine and stylosin.
Conclusion: These fungi can be used as a substitute for the production of plant’s medicinal compounds independent of wild populations of the source plant.
کلیدواژه‌ها
Ferula ovina؛ Fungal endophytes؛ Ochroconis ferulica؛ Pithoascus persicus؛ Stylosin؛ Tschimgine
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