Topical formulations containing Copaifera duckei Dwyer oleoresin improve cutaneous wound healing | ||
| Avicenna Journal of Phytomedicine | ||
| دوره 11، شماره 2، خرداد و تیر 2021، صفحه 120-133 اصل مقاله (771.78 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2020.16821 | ||
| نویسندگان | ||
| Fernanda Gosuen Gonçalves Dias* 1؛ Lucas de Freitas Pereira1؛ Ricardo Andrade Furtado1؛ Geórgia Modé Magalhães2؛ Marina Pacheco Miguel3؛ Luis Gustavo Gosuen Gonçalves Dias4؛ Adriana Torrecilhas Jorge1؛ Cristiane dos Santos Honsho1؛ Sérgio Ricardo Ambrósio5؛ Jairo Kenupp Bastos6؛ Micaela Silva Carrijo1؛ Denise Crispim Tavares5 | ||
| 1Department of Veterinary Medicine, Universidade de Franca, Franca (SP), Brazil | ||
| 2Department of Veterinary Medicine, Instituto Federal do Sul de Minas Gerais, Muzambinho (MG), Brazil | ||
| 3Department of Veterinary Medicine, Universidade Federal de Goiás, Goiânia (GO), Brazil | ||
| 4Department of Veterinary Medicine, Universidade Estadual Paulista, Jaboticabal (SP), Brazil | ||
| 5Department of Sciences, Universidade de Franca, Franca (SP), Brazil | ||
| 6Department of Pharmacy, Universidade de São Paulo, Ribeirão Preto (SP), Brazil | ||
| چکیده | ||
| Objective: Evaluation of the healing and toxicological effects of Copaifera duckei Dwyer oleoresin (CDO). Materials and Methods: Rodents with skin lesions were divided into nine groups, including daily treatments with 1, 3 and 10% CDO, collagenase, antibiotic ointment and control groups, for 14 days. Results: Treatment with 10% CDO reduced skin edema and hyperplasia, demonstrating anti-inflammatory effect of the oil. Reduction in the wound area was observed, indicating the healing effect of CDO. Histopathological analysis showed increases in angiogenesis and re-epithelialization in animals treated with the highest concentration. On the other hand, no alterations in ulcerations, inflammatory infiltrate, hemorrhage, congestion, degeneration, percentage of collagen fibers, number of cells stained with anti-macrophage migration inhibitory factor, or density of area stained with anti-collagen I and III were found. Toxicogenetic analysis revealed no differences in micronucleus frequencies or in the ratio of polychromatic erythrocytes to total erythrocytes between treated and negative control, demonstrating the absence of genotoxicity and cytotoxicity, respectively. There was no difference in levels of liver enzymes among groups, indicating the absence of hepatotoxicity. Conclusion: Formulations of CDO exerted beneficial effects on the stages of cutaneous wound healing and are promising options for the treatment of wounds. | ||
| کلیدواژهها | ||
| Cutaneous wound healing؛ Copaifera duckei Dwyer oleoresin؛ Toxicity | ||
| مراجع | ||
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