N-acetylcysteine-loaded electrospun mats improve wound healing in mice and human fibroblast proliferation in vitro: A potential application of nanotechnology in wound care | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 12، دوره 23، شماره 12، اسفند 2020، صفحه 1590-1602 اصل مقاله (1.57 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2020.41550.11078 | ||
| نویسندگان | ||
| Ramin Seyedian1؛ Elham Shabankareh Fard2؛ Maryam Najafiasl3؛ Majid Assadi4؛ Sasan Zaeri* 1 | ||
| 1Department of Pharmacology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran | ||
| 2Department of Environmental Health Engineering, Faculty of Health, Bushehr University of Medical Sciences, Bushehr, Iran | ||
| 3Department of Chemical Engineering, School of Petroleum, Gas, and Petrochemical Engineering, Persian Gulf University, Bushehr, Iran | ||
| 4Nuclear Medicine and Molecular Imaging Research Center, Bushehr University of Medical Sciences, Bushehr, Iran | ||
| چکیده | ||
| Objective(s): N-acetylcysteine (NAC) has gained attention recently in dermatology as a unique anti-oxidant. In light of progress in nanotechnological methods, it was hypothesized that loading NAC onto nanofibers would positively affect skin wound healing. The objective of this study was to fabricate NAC-loaded electrospun mats and test their effect on wound healing in vivo and in vitro. Materials and Methods: Polyvinyl alcohol (PVA)-based mats loaded with NAC at three concentrations were electrospun and characterized in terms of physicochemical properties and drug release profile. Human fibroblast cells (in vitro) and mouse full-thickness skin wounds (in vivo) were treated with mats for 5 and 14 days, respectively. Wound area, tissue histopathology, fibroblast proliferation and cellular oxidative state were evaluated. Results: Mats containing 5% PVA/NAC showed thinner fibers with suitable physicochemical properties and a sustained drug release profile. PVA/NAC (5%) mats enhanced fibroblast proliferation and attachment in vitro. The mats resulted in significant wound closure with high levels of re-epithelialization and collagen fiber synthesis on day 14 post-surgery in vivo. The mats also reduced granulation tissue and edematous stroma to a higher extent. These findings were accompanied by a significant decrease in tissue lipid peroxidation and higher superoxide dismutase activity, which may explain how NAC improved wound healing. Conclusion: We propose an NAC-loaded nanofibrous mat that takes the advantage of a porous nanoscaffold structure to release NAC in a sustained manner. This mat may be a promising candidate for further clinical evaluation. | ||
| کلیدواژهها | ||
| Electrospun nanofiber In vitro Mouse N؛ acetylcysteine (NAC) Oxidative stress Wound | ||
| مراجع | ||
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