In vitro and in vivo evaluation of antibacterial and anti-biofilm properties of five ethnomedicinal plants against oral bacteria by TEM | ||
| Avicenna Journal of Phytomedicine | ||
| دوره 11، شماره 2، خرداد و تیر 2021، صفحه 180-189 اصل مقاله (380.4 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2020.16717 | ||
| نویسندگان | ||
| Fariba Fathi1؛ Maryam Sadrnia* 2؛ Mohammad Arjomandzadegan3؛ Hamid Reza Mohajerani1 | ||
| 1Department of biology, Arak Branch, Islamic Azad University, Arak | ||
| 2Department of Biology, Payame Noor University, Iran | ||
| 3Infectious Diseases Research Center, Arak University of Medical Sciences, Arak, Iran | ||
| چکیده | ||
| Objective: The aim of the present study was to investigate antibacterial and antibiofilm activity of a few medicinal plants against oral bacteria. Materials and Methods: Salvia officinalis, Lippie citriodora, Mentha piperita, Echinacea purpurea and Matricaria chamomilla were extracted. Isolates from oral cavity were identified by microbiological and molecular methods. Minimum inhibitory concentration and minimum bactericidal concentration were determined by Broth microdilution method. The anti-biofilm activity of essential oils and extracts investigated and as a mixture by Broth dilution method. Toxicity of the herbal mixture was assayed by in Wistar rats treated with intradermal injection. Wound healing properties of the herbal mixture against infected wounds on the back of the rats were investigated. Anti-biofilm activity was investigated on tooth surfaces. Bacterial structure changes and fine- structure study were performed by light microscopy and Transmission electron microscopy. Results: The lowest MIC and MBC for the plant mixtures was 0.0002 mg/ml belonged to Streptococcus pyogenes and the highest values (0.025 mg/ml) belonged to Eikenella corrodens. The essential oils of S. officinalis, L. citriodora and M. piperita, but not E. purpurea and M. chamomilla extracts, were able to remove the biofilms created by the studied bacteria. The herbal mixture was able to completely heal the wound skin of rats in 21 days (p Conclusion: It was concluded that the essential oils of S. officinalis, L. citriodora and M. piperita had significant effects on inhibition of oral bacteria biofilm formation. | ||
| کلیدواژهها | ||
| Ethnomedicinalplants؛ Antibacterial؛ Anti-biofilm؛ Oral bacteria | ||
| مراجع | ||
|
Aghili k, Mohammad H.2003. Tehran Reservoir, Scientific and Cultural Publications 1367. AraziS. 2003. Effects of hydro alcholichydroalcholic extract of chamomile. In the prevention of nicotininduced seizures in rats.J Babol Univ Medsci, 2: 7-12. Aromatic F, Shams A, Mohammad R.2014. Herbal Treatment Guide. Tehran: Institute Med Sci Islam Repub Iran, 91: 25-3011. Bauer R, Khan IA, Wanger H. 1988, TLC and HPLC analysis of Echinacea pallida and Echinacea angustifolia roots. Planta Med, 54: 426-30. Chen F, Wang D. 2010, Novel technologies for the prevention and treatment of dental caries: a patent survey. Exp opin therap patent, 20: 94-681. Chevallier A. Encyclopedia of medicinal plants. Ttranslator: Zarezadeh A. Tehran: Vesal, 2003 Chi DL. 2013. Reducing Alaska Native paediatric oral health disparities: a systematic review of oral health interventions and a case study on multilevel Anti-biofilm properties of ethnomedicinal plants against oral bacteria AJP, Vol. 11, No. 2, Mar-Apr 2021 189 strategies to reduce sugar-sweetened beverage intake. Int J Circumpolar Health, 5: 72-210. Cockerill FR, 2006, Clinical, Institute LS. Performance standards for antimicrobial disk susceptibility testing: approved standard: National Committee for Clinical Laboratory Standards, 29: 1-76. Coffey B, Anderson G. 2014. Biofilm formation in the 96-well microtiter plate. Methods Mol Biol, 1149: 631-641. Das K, Tiwari R. Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. Med Plant Res, 4: 104-111. Ferreira I, Vidal CL, Botelho AL, Ferreira PS, Valente M, Schiavon M. 2020. Effect of nanomaterial incorporation on the mechanical and microbiological properties of dental porcelain. J Prosthet Dent, 157: 35- 43. Freire JMO, Chaves HV, Teixeira AH, de Sousa LHT, Pinto IR. 2019. Protective effect of Platymiscium floribundum Vog. in tree extract on periodontitis inflammation in rats. PLoS ONE 14: e0223800. Fazel N. 2004. Effect of supermint oil (peppermint) on pain after cesarean. J Babol Univ Sci, 25:28-33 Galati G. and O'Brien, 2004, Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radic Biol Med, 37: p. 287–303. Ghasemi Y, Rasoul-Amini S, Naseri A, MontazeriNajafabady N, Mobasher M, Dabbagh F. 2012. Microalgae biofuel potentials (Review). J biochem microbiol, 48: 126-144 Giorgio S, Pintore M, Pascale B, Bradesi K, Claudia Y, Juliano N. 1997. Chemical composition and antimicrobial activity of Rosmarinus officinalis L. oils from Sardinia and Corsica. Flav Frag, 17:15-9. Hu C, Kitts D. 2000. Studies on the antioxidant activity of Echinacea root extract. J Agric Food Chem, 48: 1466-72. Iauk L, Lbue A, Milazzo I, Rapisarda A, Blandino G. 2003 .Antibacterial activity of medicinal plant extracts against periodontopathic bacteria. Phytother Res, 17: 599-604. Khalessi A, Pack A, Thomson W, Tompkins G. 2004. An in - vivo study of the plaque control efficacy of persica TM: A commercially available herbal mouthwash coutaining extracts of Salvadora Persica. Inter Dent JOct, 54: 279-283. Khokhini B, Abu B, Hedayat K.2000.Antioxidant and Antibacterial Activities of Six Medicinally Important Species of the Genus Salvia from North East of Iran. J. Genet Resour,2: 41-47. Lang M, Rodrigues S, Boulho R, Duteil E, Bazire A, Bedoux G.2016. An Essential Oil Blend Prevents Pseudomonas aeruginosa PA01 from Forming Biofilms. J Bacteriol Parasitol,7: 268-1000. Mohammed H, Abdel-Aziz M, Hegazy M.2019. Anti-Oral Pathogens of Tecoma stans (L.) and Cassia javanica (L.) Flower Volatile Oils in Comparison with Chlorhexidine in Accordance with Their Folk Medicinal Uses, Medicina (KaunasJun 24; 55(6). pii: E301. Niculae M, Spînu M, Şandru CD, Brudaşcă F, Cadar D, SZAKACS B.2009. Antimicrobial potential of some Lamiaceae essential oils against animal multiresistant bacteria. Lucrări Scintifice Medicină Veterinară Timoara, 42: 170-175. Nurizadeh E, Mirzapur T, Ghasemi K, Razavi SM, Latifi S. 2004. The antibacterial effects of and licorice on Helicobacter pylori. J Daneshvar Shahed Univ Sci, 11: 67-72. Shafiei Z, Haji A, Rahim Z, Philip K, Thurairajah N. 2016. Antibacterial and antiadherence effects of a plant extract mixture (PEM) and its individual constituent extracts (Psidium sp., Mangifera sp., and Mentha sp.) on single- and dual-species biofilms.J Archives of Oral Biology. 5: 106-110. Sokmen A, Jones B, Erturk M. 1999. The invitro antibacterial activity of Turkish medicinal plants. J Ethnopharmacol, 67: 79- 86. Ulloa-Urizar G, Aguilar-Luis M, Dama-Odría M, Camarena-Lizarzaburu J, delalle L. 2015.Antibacterial activity of five Peruvian medicinal plants against Pseudomonas aeruginosa. Asian Pac J Trop Biomed; 5: 928-931. Vahidi A, Dashti M, Jamaledini SH.1986. effect of extract of chamomile in reducing pain in rats. J ShahidSadooghi Univ Sci, 9: 60-65. | ||
|
آمار تعداد مشاهده مقاله: 10,465 تعداد دریافت فایل اصل مقاله: 2,382 |
||