Phytochemical standardization, formulation and evaluation of oral hard gelatin capsules from Pinus eldarica bark extract | ||
| Avicenna Journal of Phytomedicine | ||
| دوره 11، شماره 2، خرداد و تیر 2021، صفحه 168-179 اصل مقاله (807.24 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2020.16716 | ||
| نویسندگان | ||
| Sajad Esmaeili1؛ Ladan Dayani2؛ Azade Taheri2؛ Behzad Zolfaghari* 3 | ||
| 1Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran. | ||
| 2Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran. | ||
| 3Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| چکیده | ||
| Objective:The extract of Pinus eldarica bark contains many polyphenolic compounds that were studied due to their high antioxidant, anti-inflammatory and anti-mutagenic effects. Therefore, the purpose of the present study was to conduct phytochemical standardization and develop hard gelatin capsules from the extract of P. eldarica bark. Materials and Methods:Extraction was carried out by maceration method at room temperature for 72 hr using ethanol 70% followed by freeze drying. Quantification and standardization tests were performed using Folin-Ciocalteu method. Then, nine formulations were prepared containing different amounts of stearic acid (1-3%) and corn starch (3%, 10%, and 25%). Each formulation was characterized by FTIR and pharmacopoeial tests such as drug content, disintegration time, flowability parameters and drug release percent. The optimized formulation underwent stability studies at 75±5% humidity and 40±2°C. Results:The total phenolic content of the extract in terms of gallic acid equivalent was 362.8±5.4 mg/g and the total procyanidin content in the extract was 174.386±2.5 mg/g. FTIR revealed no interaction between the components. The results presented that the best formulation of the capsules was achieved they contained 3% of stearic acid and 25% of corn starch. This formulation showed 91.69±0.33% of drug content, 9.36±0.02 min disintegration time and 83.02±0.81% release percent. Moreover, it showed good flowability. Stability studies on the optimized formulation displayed that the formulation was stable within 6 months in the accelerated condition. Conclusion: In conclusion, results of the present phytopharmaceutical evaluations confirmed this product as a promising herbal capsule formulation. | ||
| کلیدواژهها | ||
| Pinus eldarica؛ Capsules؛ Plant Extracts؛ Phytochemical؛ Standardization | ||
| مراجع | ||
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Afsharypuor S, Sanaty F. 2005. Essential Oil Constituents of Leaves and Fruits of Pinus eldarica Medw.J Essent Oil Res, 17:327- 328. Anbu A, Samuel JSJ. 2018. Formulation and Evaluation of Herbal Capsules Containing Dried Ethanol Extract of Gnetum gnemon Fruits. Int J Pharmacogn Phytochem Res, 12:273-280. Babaee F, Safaeian L, Zolfaghari B, Haghjoo Javanmard SH. 2016. Cytoprotective Effect of Hydroalcoholic Extract of Pinus eldarica Bark against H2O2-Induced Oxidative Stress in Human Endothelial Cells. Iran Biomed J, 20:161-167. Bankoti K, Rana M, Bharadwaj M. 2012. Accelerated stability study of herbal capsules. IOSR-PHR, 2:1-6. Bolandghamat S, Moghimi A, Iranshahi M. 2011. Effects of ethanolic extract of pine needles (Pinus eldarica Medw.) on reserpine-induced depression-like behavior in male Wistar rats. Pharmacogn Mag, 7:248-253. Carvalho HdO, Medeiros BJL, de Sá BM, C de Araújo JT, Kawakami MYM, Favacho HAS, Carvalho JCT. 2013. Study of dissolution profiles and desintegration of capsules containing the dried hydroethanolic extract of Calophyllum Esmaeili et al. AJP, Vol. 11, No. 2, Mar-Apr 2021 178 brasiliense. Rev Bras Farmacogn, 23:194- 199. Faridi P, Roozbeh J, Mohagheghzadeh A. 2012. Ibn-Sina's Life and Contributions to Medicinal Therapies of Kidney Calculi. Iran J Kidney Dis, 6:339-45. Ghadirkhomi A, Safaeian L, Zolfaghari B, Agha Ghazvini MR, Rezaei P. 2016. Evaluation of acute and sub-acute toxicity of Pinus eldarica bark extract in Wistar rats. Avicenna J Phytomed, 6:558-566. Hosseinzadeh H, Khooei AR, Khashayarmanesh Z, Motamed-Shariaty V. 2010. Antiurolithiatic activity of Pinus eldarica medw: fruits aqueous extract in rats. Urol J, 7: 232-237. Huseini HF, Anvari MS, Khoob YT, Rabbani S, Sharifi F, Arzaghi SM, Fakhrzadeh H. 2015. Anti-hyperlipidemic and antiatherosclerotic effects of Pinus eldarica Medw. nut in hypercholesterolemic rabbits. DARU, 23:32-37. Iravani S, Zolfaghari B. 2011. Pharmaceutical and nutraceutical effects of Pinus pinaster bark extract. Res Pharmaceut Sci, 6:1-11. Iravani S, Zolfaghari B. 2013. Green Synthesis of Silver Nanoparticles Using Pinus eldarica Bark Extract. Biomed Res Int, 2013:1-6. Iravani S, Zolfaghari B. 2014. Phytochemical analysis of Pinus eldarica bark. Res Pharmaceut Sci, 9: 243-250. Maimoona A, Naeem I, Saddiqe Z, Jameel K. 2011. A review on biological, nutraceutical and clinical aspects of French maritime pine bark extract. J Ethnopharmacol, 133: 261- 277. Prathyusha C, Tegk M. 2013. Compatibility studies of Donepezil with different excipients by using HPLC and FTIR. J Adv Pharmacy Edu Res, 3:273-279. New Hope Network. 2020. Pycnogenol Supplier Horphag Research Launches Nutritional Ingredient Division. [online] Available at: <https://www.newhope.com/supply-newsamp-analysis/pycnogenol-supplierhorphag-research-launches-nutritionalingredient-divisi> [Accessed 13 August 2020]. Rohdewald P. 2002. A review of the French maritime pine bark extract (Pycnogenol), a herbal medication with a diverse clinical pharmacology. Int J Clin Pharmacol Ther, 40: 158-168. Rohdewald PJ. 2015. Update on the clinical pharmacology of Pycnogenol(R). Med Res Arch, 3:1-11. Sadeghi M, Zolfaghari B, Jahanian-Najafabadi A, Abtahi SR. 2016. Anti-pseudomonas activity of essential oil, total extract, and proanthocyanidins of Pinus eldarica Medw. bark. Res Pharmaceut Sci, 11:58-64. Sarvmeili N, Jafarian-Dehkordi A, Zolfaghari B. 2016. Cytotoxic effects of Pinus eldarica essential oil and extracts on HeLa and MCF7 cell lines. Res Pharmaceut Sci, 11:476- 483. Simpson T, Kure C, Stough C. 2019. Assessing the Efficacy and Mechanisms of Pycnogenol(®) on Cognitive Aging From In Vitro Animal and Human Studies. Front Pharmacol, 10: 694-694. Singleton VL, Orthofer R, Lamuela-Raventós RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Method Enzymol, 299:152-78. Tayarani-Najaran Z, Yazdian-Robati R, Amini E, Salek F, Arasteh F, Emami A. The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death. Avicenna J Phytomed, 2019; 9: 491-498. The United States Pharmacopeia, United States Pharmacopeial Convention, Inc. official from May 1, 2007. Maritime Pine Bark Extract – USP 30 NF25. P. 964-966. The United States Pharmacopeia, United States Pharmacopeial Convention, Inc. official from May 1, 2019. General chapter volume 1– USP 42 NF37. Uddin MS, Al Mamun A, Rashid M, Asaduzzaman M. 2016. In-process and finished products quality control tests for pharmaceutical capsules according to pharmacopoeias. Brit J Pharmaceut Res, 9:1-9. Unit P, World Health Organization. WHO guidelines on stability testing of pharmaceutical products containing wellestablished drug substances in conventional dosage forms. Geneva: World Health Organization; 1996.WHO Technical Report Series, No. 863, Annex 5. Vyas T, Dash RP, Anandjiwala S, Nivsarkar M. 2011. Formulation and pharmacokinetic evaluation of hard gelatin capsule encapsulating lyophilized Vasa Swaras for improved stability and oral bioavailability of Preparation of oral Pinus eldarica bark extract capsules AJP, Vol. 11, No. 2, Mar-Apr 2021 179 vasicine. Fitoterapia, 82:446-453. Waterhouse, AL. 2002. Determination of Total Phenolics. Current Protocol Food Analytic Chem, 6:111-118. | ||
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