Survey of various carbapenem-resistant mechanisms of Acinetobacter baumannii and Pseudomonas aeruginosa isolated from clinical samples in Iran | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 5، دوره 23، شماره 11، بهمن 2020، صفحه 1396-1400 اصل مقاله (604.67 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2020.44853.10463 | ||
| نویسندگان | ||
| Leila Azimi1؛ Fatemeh Fallah1؛ Abdollah Karimi1؛ Mehdi Shirdoust1؛ Taher Azimi2؛ Iraj Sedighi Sedighi3؛ Mohammad Rahbar4؛ Shahnaz Armin* 1 | ||
| 1Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran | ||
| 2Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran | ||
| 3Pediatric department, faculty of medicine, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 4Department of Microbiology, Reference Health Laboratories Research Center, Ministry of Health and Medical Education, Tehran, Iran | ||
| چکیده | ||
| Objective(s): Pseudomonas aeruginosa and Acinetobacter baumannii resist antibiotics by different intrinsic and acquired mechanisms. This study aims to define various carbapenem-resistant mechanisms of isolated P. aeruginosa and A. baumannii from nine different provinces of Iran. Materials and Methods: In this cross-sectional study, all carbapenem-resistant P. aeruginosa and A. baumannii samples from nine provinces of Iran on a one-year time horizon were gathered. Modified Hedge Test (MHT) and Carba NP-Test were applied to the identification of producing-carbapenemase strains. The most important carbapenemase genes recognized by PCR and gene overexpression of the efflux pump were surveyed by efflux pump inhibitors (EPIs) and confirmed by Real-Time PCR. Results: Twenty-one percent and 43.5% of P. aeruginosa and A. baumannii isolates were resistant to carbapenem, respectively. MHT and Carba-NP tests identified 21% and 11% carbapenemase-producing strains in these Gram-negative bacteria, respectively. NDM-1 was the most prevalently detected carbapenemase in P. aeruginosa; OXA-51 and OXA-23 were the most significant genes in A. baumannii. EPIs identified active efflux pumps in 20% and 28% of P. aeruginosa and A. baumannii, respectively. Real-time PCR confirmed gene overexpression of efflux pumps in 54% and 30% of positive EPIs in P. aeruginosa and A. baumannii, respectively. Conclusion: P. aeruginosa and A. baumannii may become multi-drug-resistant (MDR) and Extensively Drug-Resistant (XDR) strains and cause a high rate of mortality and morbidity. Thus, it is of necessity to prohibit the spread of antibiotic-resistant strains in hospitals. | ||
| کلیدواژهها | ||
| Acinetobacter baumannii؛ Carbapenems؛ Drug resistance؛ Iran؛ Pseudomonas aeruginosa | ||
| مراجع | ||
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