Human amniotic membrane mesenchymal stem cells-conditioned medium attenuates myocardial ischemia-reperfusion injury in rats by targeting oxidative stress | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 12، دوره 23، شماره 11، بهمن 2020، صفحه 1453-1461 اصل مقاله (664.42 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2020.47572.10952 | ||
| نویسندگان | ||
| Behnaz Mokhtari1، 2؛ Yaser Azizi1، 2؛ Aliakbar Rostami Abookheili3؛ Nahid Aboutaleb* 1، 2؛ Donya Nazarinia4؛ Nasim Naderi5 | ||
| 1Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran | ||
| 2Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 3Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran | ||
| 4Department of Physiology, School of Paramedical Sciences, Dezful University of Medical Sciences, Dezful, Iran | ||
| 5Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Objective(s): Ischemic heart diseases (IHD) are one of the major causes of death worldwide. Studies have shown that mesenchymal stem cells can secrete and release conditioned medium (CM) which has biological activities and can repair tissue injury. This study aimed to investigate the effects of human amniotic membrane mesenchymal stem cells (hAMCs)-CM on myocardial ischemia/reperfusion (I/R) injury in rats by targeting oxidative stress. Materials and Methods: Male Wistar rats (40 rats, weighing 200–250 g) were randomly divided into four groups: Sham, myocardial infarction (MI), MI + culture media, and MI + conditioned medium. MI was induced by ligation of the left anterior descending coronary artery for 30 min. After 15 min of reperfusion, intramyocardial injections of hAMCs-CM or culture media (150 μl) were performed. At the end of the experiment, serum levels of cardiac troponin-I (cTn-I), myocardial levels of malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx), as well as cardiac histological changes were evaluated. Results: HAMCs-CM significantly decreased cTn-I and MDA levels and increased SOD and GPx activities (p <0.05). In addition, hAMCs-CM improved cardiac histological changes and decreased myocardial injury percentage (p <0.05). Conclusion: This study showed that hAMCs-CM has cardioprotective effects in the I/R injury condition. Reduction of oxidative stress by hAMCs-CM plays a significant role in this context. Based on the results of this study, it can be concluded that hAMCs-CM can be offered as a therapeutic candidate for I/R injury in the future, but more research is needed. | ||
| کلیدواژهها | ||
| Conditioned medium Ischemic heart diseases Mesenchymal stem cells Myocardial ischemia/؛ reperfusion injury Oxidative stress | ||
| مراجع | ||
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