Characterization of the first highly predatory Bdellovibrio bacteriovorus from Iran and its potential lytic activity against principal pathogenic Enterobacteriaceae | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 5، دوره 23، شماره 10، دی 2020، صفحه 1275-1285 اصل مقاله (1.38 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2020.43159.10146 | ||
| نویسندگان | ||
| Salman Odooli1؛ Rasoul Roghanian* 1؛ Giti Emtiazi1؛ Milad Mohkam2؛ Younes Ghasemi2، 3 | ||
| 1Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, Iran | ||
| 2Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 3Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| چکیده | ||
| Objective(s): Bdellovibrio-and-like organisms (BALOs) are predatory prokaryotes that attack and kill other Gram-negative bacteria for growth and reproduction. This study describes the isolation, identification, biological properties, and bacteriolytic activity of the first Bdellovibrio bacteriovorus with a broad prey range from Iran. Materials and Methods: One BALO strain with high predatory potency was isolated from the rhizosphere soil using Enteropathogenic Escherichia coli as prey. It was identified and designated as Bdellovibrio bacteriovorus strain SOIR-1 through plaque assays, transmission electron microscopy (TEM), Bdellovibrio-specific PCRs, and 16S rRNA gene sequence analysis. Biological characterization and analysis of bacteriolytic activity were also performed. Results: TEM and Bdellovibrio-specific PCRs confirmed that the strain SOIR-1 belongs to the genus Bdellovibrio. Analysis of the 16S rRNA gene sequence revealed its close phylogenetic relationship with strains of Bdellovibrio bacteriovorus. The strain SOIR-1 grew within the temperature range of 25–37 °C and the pH range of 6.0–8.0, with the optimal predatory activity at 30 °C and pH 7.4. It had the highest and lowest bacteriolytic activity toward Shigella dysenteriae and Pseudomonas aeruginosa with a killing rate of 89.66% and 74.83%, respectively. Conclusion: Considering the hypothesis of bdellovibrios heterogeneity, identification of new isolates contributes to a deeper understanding of their diversity, their ecological roles, and their promising potential as living antibiotics or biocontrol agents. Bdellovibrios with broad bacteriolytic nature has not previously been reported in sufficient detail from Iran. The results of this study showed the great potential of native B. bacteriovorus strain SOIR-1 in the control and treatment of diseases caused by pathogenic Enterobacteriaceae. | ||
| کلیدواژهها | ||
| 16S rRNA Analysis Bacteriolytic activity Bdellovibrio Enterobacteriaceae Iran Isolation Predation Transmission electron؛ microscopy | ||
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| مراجع | ||
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