Orthodontic treatment induces Th17/Treg cells to regulate tooth movement in rats with periodontitis | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 10، دوره 23، شماره 10، دی 2020، صفحه 1315-1322 اصل مقاله (782.93 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2020.44437.10419 | ||
| نویسندگان | ||
| Nan Ge1، 2؛ Jing Peng1، 3؛ Lan Yu1، 4؛ Shuo Huang1؛ Lu Xu1؛ Ying Su5؛ Li Chen* 1 | ||
| 1Department of Orthodontics, Beijing Stomatological Hospital & School of Stomatology, Capital Medical University, Beijing 100050, China | ||
| 2Department of Stomatology, Beijing Pinggu Hospital, Beijing 101200, China | ||
| 3Department of Orthodontics, Stomatological Hospital of Guangzhou Medical University, Guangzhou 100079, China | ||
| 4Department of Stomatology, Tie Ying Hospital of Fengtai District Beijing, Beijing 100079, China | ||
| 5Institute of dental Research, Beijing Stomatological Hospital & School of Stomatology, Capital Medical University, Beijing 100050, China | ||
| چکیده | ||
| Objective(s): Here we investigated the regulation of Th17 and Treg cells in orthodontic tooth movement during periodontal inflammation. Materials and Methods: Fifty-six SD rats were divided into a control (24 rats) and a tooth movement group during the recovery stage of periodontitis (RM group, 32 rats). Periodontitis was established by silk ligation and local injection of LPS. Orthodontic tooth movement was achieved by nickel-titanium springs on the maxillary first molars. The proportions of Th17 cells and Treg cells were evaluated by flow cytometry. Gene expression of ROR-γt and Foxp3 was determined by real-time PCR. Expression of ROR-γt, Foxp3, RANK, RANKL, and OPG was detected by immunohistochemical staining. Osteoclasts were detected by TRAP staining. Relationships between Th17/Treg cells, osteoclasts, and related factors were estimated by correlation and regression analysis. Results: During orthodontic tooth movement in the recovery stage of periodontitis, the proportion of Th17 cells, ROR-γt, RANK, osteoclasts, and the RANKL/OPG ratio increased and then decreased. The proportion of Treg cells and Foxp3 increased, then decreased, and increased again. Levels of RANKL and OPG increased, then decreased, then increased, and finally decreased. The Th17/Treg ratio initially decreased, then increased, and decreased again. Th17 cells were positively correlated with RANK and RANKL, the RANKL/OPG ratio, and counts of osteoclasts. Treg cells were negatively correlated with RANK expression and numbers of osteoclasts. The Th17/Treg ratio was positively correlated with RANK expression and numbers of osteoclasts. Conclusion: Under periodontal inflammation conditions, the Th17/Treg ratio might regulate orthodontic tooth movement through changing osteoclasts metabolism. | ||
| کلیدواژهها | ||
| Orthodontics؛ Osteoclasts؛ Periodontitis؛ Th17 cells؛ Treg cells | ||
| مراجع | ||
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