Effects of ethyl acetate extract of Salsola collina on brain-gut peptides and interstitial cells of gastric Cajal in rats with diabetic gastroparesis | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 14، دوره 23، شماره 9، آذر 2020، صفحه 1218-1224 اصل مقاله (672.37 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2020.43521.10223 | ||
| نویسندگان | ||
| Xinling Zhao1؛ Hongbo Wang2؛ Ziwei Zhang1؛ Hong Jin1؛ Yanling Gong* 1 | ||
| 1Department of Pharmacy, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, Shandong, China | ||
| 2Department of Gastroenterology surgery, Jimo People’s Hospital, Qingdao, Shandong, China | ||
| چکیده | ||
| Objective(s): Effects of ethyl acetate extract of Salsola collina (EES) on brain-gut peptides and interstitial cells of gastric Cajal in rats with diabetic gastroparesis were explored. Materials and Methods: Rats were divided into six groups: normal control group (NC), diabetic gastroparesis model group (DGP), low, medium, and high dose of EES groups (LES, MES, and HES, respectively), and metoclopramide positive group (MPG). DGP rats were induced by streptozotocin (STZ) combined with a high-sugar-high-fat diet. The gastric emptying was measured by the phenol red labeling method. Enzyme-linked immunosorbent assay (ELISA) was employed to determine the concentrations of serum ghrelin, gastrin (GAS), somatostatin (SS), and vasoactive intestinal peptide (VIP). The expressions of c-Kit and its natural ligand stem cell factor (SCF) in gastric tissues were determined by Western blot and immunofluorescence. Results: Gastric emptying rate increased in a different degree after intervention by EES, among which MES and HES groups showed a significant effect (compared with DGP, P<0.01) and the HES group was equivalent to the MPG group; serum ghrelin and content of serum GAS increased while SS and VIP decreased (compared with the DGP group, PConclusion: EES significantly improved gastric emptying by regulating gastrointestinal hormone excretion and c-Kit/SCF signaling pathway. Our study provides a pharmacological basis for the use of the EES in the treatment of DGP. However, the detailed molecular mechanism remains to be clarified. | ||
| کلیدواژهها | ||
| Brain؛ gut peptides Cajal interstitial cells Diabetic gastroparesis Ethyl acetate extract Gastric motility Salsola collina | ||
| مراجع | ||
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