Protective effect of crocin on bisphenol A - induced spatial learning and memory impairment in adult male rats: Role of oxidative stress and AMPA receptor | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 6، دوره 23، شماره 9، آذر 2020، صفحه 1146-1154 اصل مقاله (742.68 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2020.41097.9714 | ||
| نویسندگان | ||
| Faezeh Vahdati Hassani1؛ Elaheh Masjedi1؛ Hossein Hosseinzadeh2، 1؛ Zeinab Bedrood1؛ Khalil Abnous2؛ Soghra Mehri* 2، 1 | ||
| 1Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 2Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| چکیده | ||
| Objective(s): Bisphenol A (BPA), a xenoestrogenic endocrine disrupting agent, is widely used in the production of polycarbonate plastics and has potential adverse effects on the developing nervous system, memory and learning abilities. The protective effect of the crocin, an important active constituent in Crocus sativus L, on memory impairment induced by BPA in rat was determined through evaluation of oxidative stress and the level of NMDA (N-methyl-D-aspartate receptors) and AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazole-propionicd acid) receptors. Materials and Methods: Rats were orally treated with BPA (100 mg/kg) or sesame seed oil in control group for 28 days. Crocin (10, 20, and 40 mg/kg, IP) was administrated in BPA-orally treated groups for 28 days. Memory and learning functions were evaluated by Morris water maze. The level of malondialdehyde (MDA) and glutathione (GSH) contents were determined in rat hippocampus. Additionally, the expression of NMDA and AMPA receptors were analyzed using Western blot method. Results: Administration of BPA significantly reduced memory and learning functions. Crocin significantly protected against learning and memory impairments induced by BPA. BPA administration markedly reduced GSH content and induced lipid peroxidation, while crocin was able to increase GSH content in rat hippocampus. The expression of NMDA receptor did not change in BPA-treated rats, while the significant reduction in AMPA receptor expression was observed. Moreover, crocin (20 mg/kg) significantly elevated the expression of AMPA receptor. Conclusion: Crocin recovered spatial learning and memory defects induced by BPA in part through anti-oxidant activity and modulation the expression of AMPA receptor in rat hippocampus. | ||
| کلیدواژهها | ||
| Crocin Crocus sativus L. Bisphenol A Anti؛ oxidant AMPA NMDA | ||
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| مراجع | ||
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