Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 2، دوره 3، شماره 2، خرداد 2013، صفحه 112-125 اصل مقاله (319.28 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2013.16 | ||
| نویسندگان | ||
| Badrul Alam1؛ Fahima Akter1؛ Nahida Parvin1؛ Rashna Sharmin Pia1؛ Sharmin Akter1؛ Jesmin Chowdhury1؛ Kazi Sifath-E-Jahan1؛ Ekramul Haque Haque* 2 | ||
| 1Department of Pharmacy, Atish Dipankar University of Science & Technology, Dhaka, Bangladesh | ||
| 2Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh | ||
| چکیده | ||
| Objective:The present study was designed to evaluate the antioxidant, analgesic, and anti-inflammatory activities ofthe methanolic extract of Piper betle leaves (MPBL). Materials and Methods: MPBL was evaluated for anti-inflammatory activity using carrageenan-induced hind paw edema model. Analgesic activity of MPBL was evaluated by hot plate, writhing, and formalin tests. Total phenolic and flavonoids content, total antioxidant activity, scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, peroxynitrate (ONOO-) as well as inhibition of total ROS generation, and assessment of reducing power were used to evaluate antioxidant potential of MPBL. Results: The extract of MPBL, at the dose of 100 and 200 mg/kg, produced a significant (p<0.05) increase in pain threshold in hot plate method whereas significantly (p<0.05) reduced the writhing caused by acetic acid and the number of licks induced by formalin in a dose-dependent manner. The same ranges of doses of MPBL caused significant (p<0.05) inhibition of carrageenan-induced paw edema after 4 h in a dose-dependent manner. In DPPH, ONOO-, and total ROS scavenging method, MPBL showed good antioxidant potentiality with the IC50 value of 16.33±1.02, 25.16±0.61 , and 41.72±0.48 µg/ml, respectively with a significant (p<0.05) good reducing power. Conclusion: The findings of the study suggested that MPBLhas strong analgesic, anti-inflammatory, and antioxidant effects, conforming the traditional use of this plant for inflammatory pain alleviation to its antioxidant potentiality. | ||
| کلیدواژهها | ||
| Analgesic؛ Antioxidant؛ Anti-inflammatory؛ Piper betle | ||
| مراجع | ||
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