سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Kalalinia, Fatemeh, Amiri, Nafise, Mehrvarzian, Niloufar, Fazly Bazzaz, Bibi Sedigheh, Iranshahi, Mehrdad, Shahroodi, Azadeh, Arabzadeh, Sepideh, Abbaspour, Mohammadreza, Badiee Aaval, Shapour, Movaffagh, Jebrail. (1399). Topical green tea formulation with anti-hemorrhagic and antibacterial effects. , 23(8), 1085-1090. doi: 10.22038/ijbms.2020.41397.9782
Fatemeh Kalalinia; Nafise Amiri; Niloufar Mehrvarzian; Bibi Sedigheh Fazly Bazzaz; Mehrdad Iranshahi; Azadeh Shahroodi; Sepideh Arabzadeh; Mohammadreza Abbaspour; Shapour Badiee Aaval; Jebrail Movaffagh. "Topical green tea formulation with anti-hemorrhagic and antibacterial effects". , 23, 8, 1399, 1085-1090. doi: 10.22038/ijbms.2020.41397.9782
Kalalinia, Fatemeh, Amiri, Nafise, Mehrvarzian, Niloufar, Fazly Bazzaz, Bibi Sedigheh, Iranshahi, Mehrdad, Shahroodi, Azadeh, Arabzadeh, Sepideh, Abbaspour, Mohammadreza, Badiee Aaval, Shapour, Movaffagh, Jebrail. (1399). 'Topical green tea formulation with anti-hemorrhagic and antibacterial effects', , 23(8), pp. 1085-1090. doi: 10.22038/ijbms.2020.41397.9782
Kalalinia, Fatemeh, Amiri, Nafise, Mehrvarzian, Niloufar, Fazly Bazzaz, Bibi Sedigheh, Iranshahi, Mehrdad, Shahroodi, Azadeh, Arabzadeh, Sepideh, Abbaspour, Mohammadreza, Badiee Aaval, Shapour, Movaffagh, Jebrail. Topical green tea formulation with anti-hemorrhagic and antibacterial effects. , 1399; 23(8): 1085-1090. doi: 10.22038/ijbms.2020.41397.9782

Topical green tea formulation with anti-hemorrhagic and antibacterial effects

Iranian Journal of Basic Medical Sciences
مقاله 16، دوره 23، شماره 8، آبان 2020، صفحه 1085-1090    اصل مقاله (584.08 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.41397.9782
نویسندگان
Fatemeh Kalalinia1؛ Nafise Amiri2؛ Niloufar Mehrvarzian2؛ Bibi Sedigheh Fazly Bazzaz1، 3؛ Mehrdad Iranshahi1؛ Azadeh Shahroodi4، 5؛ Sepideh Arabzadeh1؛ Mohammadreza Abbaspour4، 5؛ Shapour Badiee Aaval6؛ Jebrail Movaffagh* 4، 5
1Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
2Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
4Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
5Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
6Complementary Medicine Research Center, Faculty of Traditional Medicine, Mashhad University of Medical Sciences, Mashhad, lran
چکیده
Objective(s): Potentially preventable death from uncontrolled hemorrhage clearly indicates the importance of simple, fast and efficient ways to achieving hemostasis. The aim of this study was to develop a topical formulation of green tea extract for reducing bleeding that can be helpful in hemorrhage control.
Materials and Methods: Hydroalcoholic extract of green tea was isolated from Camellia sinensis and formulated in polyvinyl alcohol (PVA) to achieve two concentrations of 2% and 4% v/v. Folin-Ciocalteau assay was used to determine the total amount of tannins in extract. Rheological behavior of solutions was investigated by measuring viscosity at shear rates of 0–200 sec−1. Quantitative and qualitative microbial limit tests and minimum inhibitory concentration (MIC) assay were done. The effect of formulations on bleeding time was evaluated in an animal model.
Results: The total amount of tannin in green tea extract was 3.8% w/w and addition of green tea significantly increased the viscosity of PVA. The results of MIC assay showed that PVA could not inhibit the growth of bacteria, while, 716 µg/ml of green tea and 2860 µg/ml of green tea/PVA 4% inhibited the growth of Staphylococcus aureus and Pseudomonas aeruginosa. In an animal study both 2% and 4% formulations were able to stop hemorrhage approximately at an equal time compared with tranexamic acid (TXA) 50 mg/ml as a control and the lowest bleeding time was 6.4±0.51 sec for green tea/PVA 4%.
Conclusion: Based on our results, the topical formulation of green tea extract in PVA has a great potential for anti-hemorrhage applications.
کلیدواژه‌ها
Bleeding؛ Green tea؛ Hemostasis؛ Polyvinyl alcohol؛ Tannin؛ Topical formulation
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