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khodir, suzan, Al-Gholam, Marwa, Salem, Heba R.. (1399). L-Carnitine potentiates the anti-inflammatory and antinociceptive effects of diclofenac sodium in an experimentally induced knee osteoarthritis rat model. , 23(8), 1035-1044. doi: 10.22038/ijbms.2020.43136.10138
suzan khodir; Marwa Al-Gholam; Heba R. Salem. "L-Carnitine potentiates the anti-inflammatory and antinociceptive effects of diclofenac sodium in an experimentally induced knee osteoarthritis rat model". , 23, 8, 1399, 1035-1044. doi: 10.22038/ijbms.2020.43136.10138
khodir, suzan, Al-Gholam, Marwa, Salem, Heba R.. (1399). 'L-Carnitine potentiates the anti-inflammatory and antinociceptive effects of diclofenac sodium in an experimentally induced knee osteoarthritis rat model', , 23(8), pp. 1035-1044. doi: 10.22038/ijbms.2020.43136.10138
khodir, suzan, Al-Gholam, Marwa, Salem, Heba R.. L-Carnitine potentiates the anti-inflammatory and antinociceptive effects of diclofenac sodium in an experimentally induced knee osteoarthritis rat model. , 1399; 23(8): 1035-1044. doi: 10.22038/ijbms.2020.43136.10138

L-Carnitine potentiates the anti-inflammatory and antinociceptive effects of diclofenac sodium in an experimentally induced knee osteoarthritis rat model

Iranian Journal of Basic Medical Sciences
مقاله 10، دوره 23، شماره 8، آبان 2020، صفحه 1035-1044    اصل مقاله (1.81 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.43136.10138
نویسندگان
suzan khodir1؛ Marwa Al-Gholam* 2؛ Heba R. Salem1
1Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
2Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
چکیده
Objective(s): The aim of the present research is to investigate the efficacy of L-carnitine (LC) as a complementary therapy to diclofenac sodium (Dic) treatment in a mono-iodoacetate (MIA) induced knee osteoarthritis (OA) rat model, with respect to pain relief and the underlying pathology.
Materials and Methods: Fifty adult male albino rats were randomly divided into five groups (n=10): Control, OA, OA/Dic, OA/LC, and OA/Dic+LC. Knee diameter and pain assessment tests were done weekly. After four weeks, serum malondialdehyde, reduced glutathione, interleukin 1-β, tumor necrosis factor-alpha, prostaglandin E2, and bone-specific alkaline phosphatase were measured. The injected knees were removed and processed for the histological and immunohistological study of matrix metalloproteinase-13 (MMP-13) and cyclooxygenase 2 (COX-2). Also, histological examination of dorsal root ganglia and calcitonin gene-related peptide (CGRP) expression in the spinal cord were assessed.
Results: Treatment with Dic and/or LC significantly reduced knee swelling, improved pain-related behaviors, inflammatory and oxidative stress markers, attenuated the MIA-mediated histopathological alteration in the knee joint, and down-regulated expression of MMP-13 and COX-2 in the knee joint. It, also, significantly reduced CGRP expression, compared with the OA group. Dic+LC showed a better effect in improving some parameters than each treatment alone.
Conclusion: LC plus Dic is a more effective therapy than Dic alone for OA treatment.
کلیدواژه‌ها
CGRP COX؛ 2 L؛ carnitine MMP؛ 13 Osteoarthritis
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