سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Rafighdoust, Hediyeh, Ahangarzadeh, Shahrzad, Yarian, Fatemeh, Taheri, Ramezan Ali, Lari, Arezou, Bandehpour, Mojgan, Salahshoor Dahr, Mona. (1399). Bioinformatics prediction and experimental validation of VH antibody fragment interacting with Neisseria meningitidis factor H binding protein. , 23(8), 1053-1058. doi: 10.22038/ijbms.2020.44007.10318
Hediyeh Rafighdoust; Shahrzad Ahangarzadeh; Fatemeh Yarian; Ramezan Ali Taheri; Arezou Lari; Mojgan Bandehpour; Mona Salahshoor Dahr. "Bioinformatics prediction and experimental validation of VH antibody fragment interacting with Neisseria meningitidis factor H binding protein". , 23, 8, 1399, 1053-1058. doi: 10.22038/ijbms.2020.44007.10318
Rafighdoust, Hediyeh, Ahangarzadeh, Shahrzad, Yarian, Fatemeh, Taheri, Ramezan Ali, Lari, Arezou, Bandehpour, Mojgan, Salahshoor Dahr, Mona. (1399). 'Bioinformatics prediction and experimental validation of VH antibody fragment interacting with Neisseria meningitidis factor H binding protein', , 23(8), pp. 1053-1058. doi: 10.22038/ijbms.2020.44007.10318
Rafighdoust, Hediyeh, Ahangarzadeh, Shahrzad, Yarian, Fatemeh, Taheri, Ramezan Ali, Lari, Arezou, Bandehpour, Mojgan, Salahshoor Dahr, Mona. Bioinformatics prediction and experimental validation of VH antibody fragment interacting with Neisseria meningitidis factor H binding protein. , 1399; 23(8): 1053-1058. doi: 10.22038/ijbms.2020.44007.10318

Bioinformatics prediction and experimental validation of VH antibody fragment interacting with Neisseria meningitidis factor H binding protein

Iranian Journal of Basic Medical Sciences
مقاله 12، دوره 23، شماره 8، آبان 2020، صفحه 1053-1058    اصل مقاله (779.73 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.44007.10318
نویسندگان
Hediyeh Rafighdoust1؛ Shahrzad Ahangarzadeh2؛ Fatemeh Yarian* 3، 4؛ Ramezan Ali Taheri5؛ Arezou Lari6؛ Mojgan Bandehpour3؛ Mona Salahshoor Dahr7
1Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3Cellular & Molecular Biology Research Center Shahid Beheshti University of Medical Sciences, Tehran, Iran
4Department of Biotechnology, School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences, Tehran, Iran
5Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences,Tehran, Iran
6Systems Biomedicine, Pasteur Institute of Iran, Tehran, Iran
7Department of Biology, Faclty of Basic Science, Islamic Azad University Islamshahr Branch, Islamshahr, Iran
چکیده
Objective(s): We previously conducted an in silico research on the interactions between the ribosome display-selected single chain variable fragment (scFv) and factor H binding protein (fHbp) of Neisseria meningitidis. We found that heavy chain variable (VH) fragment of this scFv had considerable affinity to fHbp. These results led us to evaluate the ability of this small antibody fragment in binding and detection of fHbp antigen.
Materials and Methods: In this study, at first, the three-dimensional structure of VH fragment was simulated by Kotai Antibody Builder web server. By using ClusPro 2.0 web server, the 3D structure of the soluble form of fHbp (PDB: 2KC0) was docked to the modeled VH fragment to extract the structure of the complex’s binding. Molecular dynamics (MD) simulation was carried out using GROMACS 4.5.3 package for 65 ns. Secondly, coding sequence of VH fragment was cloned separately and expressed in Escherichia coli. After purification of the VH fragment, its binding activity to fHbp protein was analyzed by enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) method.
Results: Important amino acids involved in antigen- antibody interaction were identified by analyzing the fHbp-VH complex. The ability of the VH antibody fragment to bind and detect fHbp antigen has been confirmed by the results of in silico analysis, ELISA and SPR methods.
Conclusion: These results showed that this small fragment of antibody could be used for designing diagnostic kits.
کلیدواژه‌ها
Cloning؛ Heavy chain variable؛ Modeling؛ Molecular docking؛ Surface plasmon resonance
مراجع
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