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Morales, Jose, Lopez, Ruth, Lopez, Jorge, Lozano, Jair, Jarillo, Rosa, Flores, Hector, Castillo, Enrique. (1399). Left ventricular phosphorylation patterns of Akt and ERK1/2 after triiodothyronine intracoronary perfusion in isolated hearts and short-term in vivo treatment in Wistar rats. , 23(8), 1091-1099. doi: 10.22038/ijbms.2020.44776.10451
Jose Morales; Ruth Lopez; Jorge Lopez; Jair Lozano; Rosa Jarillo; Hector Flores; Enrique Castillo. "Left ventricular phosphorylation patterns of Akt and ERK1/2 after triiodothyronine intracoronary perfusion in isolated hearts and short-term in vivo treatment in Wistar rats". , 23, 8, 1399, 1091-1099. doi: 10.22038/ijbms.2020.44776.10451
Morales, Jose, Lopez, Ruth, Lopez, Jorge, Lozano, Jair, Jarillo, Rosa, Flores, Hector, Castillo, Enrique. (1399). 'Left ventricular phosphorylation patterns of Akt and ERK1/2 after triiodothyronine intracoronary perfusion in isolated hearts and short-term in vivo treatment in Wistar rats', , 23(8), pp. 1091-1099. doi: 10.22038/ijbms.2020.44776.10451
Morales, Jose, Lopez, Ruth, Lopez, Jorge, Lozano, Jair, Jarillo, Rosa, Flores, Hector, Castillo, Enrique. Left ventricular phosphorylation patterns of Akt and ERK1/2 after triiodothyronine intracoronary perfusion in isolated hearts and short-term in vivo treatment in Wistar rats. , 1399; 23(8): 1091-1099. doi: 10.22038/ijbms.2020.44776.10451

Left ventricular phosphorylation patterns of Akt and ERK1/2 after triiodothyronine intracoronary perfusion in isolated hearts and short-term in vivo treatment in Wistar rats

Iranian Journal of Basic Medical Sciences
مقاله 17، دوره 23، شماره 8، آبان 2020، صفحه 1091-1099    اصل مقاله (1.23 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.44776.10451
نویسندگان
Jose Morales1؛ Ruth Lopez1؛ Jorge Lopez2؛ Jair Lozano2؛ Rosa Jarillo1؛ Hector Flores3؛ Enrique Castillo* 1
1Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México, México
2Departamento de Biología Celular, Instituto Nacional de Perinatología, Ciudad de México, México
3Departamento de Inmuno-Bioquímica, Instituto Nacional de Perinatología, Ciudad de México, México
چکیده
Objective(s): To determine the effects of triiodothyronine (T3) intracoronary perfusion in isolated hearts and short-term administration in rats on the left ventricular (LV) phosphorylation patterns of Akt and ERK1/2.
Materials and Methods: Cardiodynamic and hemodynamic parameters were evaluated in Langendorff–perfused hearts. Left ventricles were used for histomorphometric and Western blot analyses. Short-term hyperthyroidism was established by T3 (500 μg.kg-1.d-1; subcutaneous injection) for 1 (T31d), 3 (T33d), and 10 (T310d) days.
Results: Isolated hearts receiving T3 perfusion did not modify LV developed pressure, +dP/dtmax, -dP/dtmin, heart rate, and coronary perfusion pressure compared with vehicle-perfused hearts. P-ERK1/2 and p-Akt levels in LV tissues after 5, 15, or 60 min of T3 or vehicle perfusion were similar. Compared with their time-matched controls, isolated hearts of T33d and T310d rats exhibited LV hypertrophy and increased absolute values of +dP/dtmax and -dP/dtmin (i.e., positive inotropic and lusitropic effects). P-ERK1/2 decreased in LV tissues of T31d and T310d but not in those of T33d rats, and p-Akt levels augmented in left ventricles of T33d and stayed unaltered in those of T31d and T310d rats.
Conclusion: T3 intracoronary perfusion did not alter cardiodynamics and hemodynamics nor influence the activation of Akt and ERK of normal hearts. Accordingly, the rapid non-genomic effects of T3 were not evident. Short-term T3 treatment provoked cardiac hypertrophy coincidental with increased LV function and associated with transient Akt activation and cyclic ERK1/2 inhibition; which implies activation of  physiological hypertrophy signaling and deactivation of  pathological hypertrophy signaling, respectively.
کلیدواژه‌ها
Akt ERK1؛ 2 Heart hypertrophy Rat Triiodothyronine treatment
مراجع
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