Evaluating the effect of arachidonic acid and eicosapentaenoic acid on induction of adipogenesis in human adipose-derived stem cells | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 9، دوره 23، شماره 8، آبان 2020، صفحه 1028-1034 اصل مقاله (846.37 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2020.41557.9819 | ||
| نویسندگان | ||
| Rezvan Mostoli1؛ Farjam Goudarzi2؛ Adel Mohamadalipoor3؛ Iraj Khodadadi1؛ Mohammad Taghi Goodarzi* 1 | ||
| 1Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 2Regenerative Medicine Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran | ||
| 3Department of Clinical Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| چکیده | ||
| Objective(s): Adipose tissue is one of the most important endocrine organs that liberates many metabolic mediators such as hormones, cytokines, and chemokines. Different types of fatty acids have key roles in adipogenesis. The aim of this study was to evaluate the effects of two essential fatty acids, including Arachidonic acid (AA) and Eicosapentaenoic acid (EPA), on the process of adipogenicity in human Adipose-Derived Stem Cells (hADSCs). Materials and Methods: After immunophenotyping of hADSCs by flowcytometry, they were differentiated into adipocytes and simultaneously exposed to 30 μM and 60 μM of AA and 25 μM and 50 μM of EPA. Further, along with the MTS assay, the activity of glycalaldehyde-3-phosphate dehydrogenase (GAPDH) was also measured. In addition, expression of lipid markers including peroxisome proliferator-activated receptor γ2 (PPARγ2) and glucose transporter 4 (GLUT4) was evaluated, and the neutral lipid contents were determined using Oil red O staining. Results: MTS evaluation showed a significant decrease in proliferation in all treatment groups compared to the control group. Based on oil red O staining, fat droplets in the AA treatment groups were higher than in controls. The expression of PPARγ2 and GLUT4 genes and proteins increased in almost all AA and EPA groups compared to control. In addition, GAPDH activity was higher in AA groups than in the control group. In general, while different concentrations of EPA did not increase the adipogenic process compared to the control group, stimulation of differentiation to adipocytes was largely determined by the AA. Conclusion: The result indicates a positive effect of omega-6 versus omega-3 in stimulating the pathways of adipogenesis. | ||
| کلیدواژهها | ||
| Adipogenesis Arachidonic acid Eicosapentaenoic acid GLUT4 Human adipose؛ derived؛ stem cell PPARγ2 | ||
| مراجع | ||
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