Preparation and characterization of bear bile-loaded pH sensitive in-situ gel eye drops for ocular drug delivery | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 13، دوره 23، شماره 7، مهر 2020، صفحه 922-929 اصل مقاله (1.17 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2020.45386.10562 | ||
| نویسندگان | ||
| Xiaomin Ni1؛ Qin Guo1؛ Yiqing Zou1؛ Yang Xuan1؛ Imran Shair Mohammad* 1؛ Qing Ding2؛ Haiyan Hu1 | ||
| 1School of Pharmaceutical Sciences, Sun Yat-sen University, University Town, Guangzhou 510006, PR China | ||
| 2Yunnan Dai Medicine Co., Ltd., Yunnan 678699, PR China | ||
| چکیده | ||
| Objective(s): In this study, a stable bear bile-loaded pH sensitive in-situ eye drop gel was prepared for sustain delivery and enhanced therapeutic application. Materials and Methods: Bear bile-loaded in-situ ocular gels with different Carbopol/Hydroxypropyl methylcellulose (HPMC) ratios were prepared and their stability was tested in PBS at a series of pH at 40 °C. The morphology was observed by SEM examination and rheology was observed by Rheometer equipped with a 60-mm cone-plate at apex angle of 1°. Gel erosion and release kinetics of Tauroursodeoxycholic acid (TUDCA) was determined by HPLC. While, the in vivo dwelling time was obtained after administering the fluorescent-loaded gel in ocular disease-free New Zealand rabbits. Finally, biocompatibility and toxicity was observed by irritation test and H&E staining of eye-ball tissues, respectively. Results: The bear bile-loaded in-situ ocular gel showed excellent stability at different pH (pH 5.0, 5.5, 6.0, 6.5, 7.0 and 8.0) up to 5 days, and bear bile extract significantly attenuated the gelling ability of the in-situ gel. The viscosity of in-situ gels formulation was decreased with increase in shear rate (0.01 to 100 s-1), and morphological examination of freeze-dried preparation showed three-dimensional reticular structure at physiological pH. The in-situ ocular gel exhibited promising sustained drug release up to 160 min in vitro, and showed prolonged retention time up to 3-folds in vivo. Finally, the biocompability data confirmed that the formulation did not induce any toxic effects and was completely compatible with eye tissues. Conclusion: pH sensitive in-situ ocular gel provides new research opportunities to efficiently treat eye diseases. | ||
| کلیدواژهها | ||
| Bear bile؛ In-situ ocular gel؛ pH sensitive؛ Rheology؛ Sustain-releas | ||
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| مراجع | ||
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