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Sobhani, Nafiseh, Sazgarnia, Ameneh, Rajabi, Omid, Soudmand, Samaneh, Naghavi, Nadia. (1391). A Study on the Photobleaching Effect of 5-ALA Conjugated Gold Nanoparticles in a CT26 Tumor Model During Photodynamic Therapy. , 9(3), 217-224. doi: 10.22038/ijmp.2012.156
Nafiseh Sobhani; Ameneh Sazgarnia; Omid Rajabi; Samaneh Soudmand; Nadia Naghavi. "A Study on the Photobleaching Effect of 5-ALA Conjugated Gold Nanoparticles in a CT26 Tumor Model During Photodynamic Therapy". , 9, 3, 1391, 217-224. doi: 10.22038/ijmp.2012.156
Sobhani, Nafiseh, Sazgarnia, Ameneh, Rajabi, Omid, Soudmand, Samaneh, Naghavi, Nadia. (1391). 'A Study on the Photobleaching Effect of 5-ALA Conjugated Gold Nanoparticles in a CT26 Tumor Model During Photodynamic Therapy', , 9(3), pp. 217-224. doi: 10.22038/ijmp.2012.156
Sobhani, Nafiseh, Sazgarnia, Ameneh, Rajabi, Omid, Soudmand, Samaneh, Naghavi, Nadia. A Study on the Photobleaching Effect of 5-ALA Conjugated Gold Nanoparticles in a CT26 Tumor Model During Photodynamic Therapy. , 1391; 9(3): 217-224. doi: 10.22038/ijmp.2012.156

A Study on the Photobleaching Effect of 5-ALA Conjugated Gold Nanoparticles in a CT26 Tumor Model During Photodynamic Therapy

Iranian Journal of Medical Physics
مقاله 8، دوره 9، شماره 3، آذر 2012، صفحه 217-224    اصل مقاله (470.02 K)
نوع مقاله: Original Paper
شناسه دیجیتال (DOI): 10.22038/ijmp.2012.156
نویسندگان
Nafiseh Sobhani1؛ Ameneh Sazgarnia* 1؛ Omid Rajabi2؛ Samaneh Soudmand1؛ Nadia Naghavi3
1Department and Research Center of Medical Physics, Mashhad University of Medical Sciences, Mashhad, Iran
2Medicinal Chemistry Department, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3Electrical Engineering Department, Faculty of Engineering, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده
Introduction
During the process photodynamic therapy (PDT), bleaching of photosensitizer induced by irradiation and generation of reactive oxygen species (ROS) can provide some information concerning the efficiency of treatment. Since gold nanoparticles (GNPs) have been highlighted as efficient drug delivery systems, in this study, by utilizing GNPs conjugated with 5 aminolevulinic acid (5-ALA-GNPs), the photobleaching of ALA-induced protoporphyrin IX (PpIX) was estimated on a colon carcinoma tumor model.
Materials and Methods
CT26 tumor models were prepared by subcutaneous injection of 5×105 cells into the right flank of Balb/c inbred mice. To estimate the time required to reach maximum concentration of PpIX in the tumors, the fluorescence signal of PpIX was monitored and PDT was performed by intratumoral injection of 5-ALA-GNPs, GNPs, and 5-ALA in separated groups for 15 min with a cycle of 5 min irradiation and 1 min darkness. The photobleaching rate was calculated from recorded fluorescence signals at the darkness intervals.
Results
The maximum fluorescence of PpIX was recorded 3 and 5 hr after injection of 5-ALA and 5-ALA-GNPs, respectively. Despite the low PpIX accumulation in tumors receiving conjugate, the photobleaching rate of PpIX was determined to be higher than 5-ALA. The reduction of the fluorescence signal due to 5-ALA-GNPs clearance was higher than that of 5-ALA.
Conclusion
Administration of 5-ALA-GNPs, intensification of ROS generating and the subsequent elevation of photobleaching results in higher treatment efficiency. Also, more rapid clearance of PpIX has an important implication in clinical application of 5-ALA-GNPs that decreases the undesirable effects on healthy tissues.
کلیدواژه‌ها
5 -ALA-Conjugated Gold Nanoparticles؛ CT26 Tumor Model؛ Photobleaching؛ Photodynamic Therapy
مراجع
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