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Soheili, Vahid, Taghdisi, Seyed Mohammad, Abnous, Khalil, Ebrahimi, Mohsen. (1399). Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensor. , 23(7), 901-908. doi: 10.22038/ijbms.2020.44016.10322
Vahid Soheili; Seyed Mohammad Taghdisi; Khalil Abnous; Mohsen Ebrahimi. "Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensor". , 23, 7, 1399, 901-908. doi: 10.22038/ijbms.2020.44016.10322
Soheili, Vahid, Taghdisi, Seyed Mohammad, Abnous, Khalil, Ebrahimi, Mohsen. (1399). 'Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensor', , 23(7), pp. 901-908. doi: 10.22038/ijbms.2020.44016.10322
Soheili, Vahid, Taghdisi, Seyed Mohammad, Abnous, Khalil, Ebrahimi, Mohsen. Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensor. , 1399; 23(7): 901-908. doi: 10.22038/ijbms.2020.44016.10322

Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensor

Iranian Journal of Basic Medical Sciences
مقاله 10، دوره 23، شماره 7، مهر 2020، صفحه 901-908    اصل مقاله (1.06 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.44016.10322
نویسندگان
Vahid Soheili1؛ Seyed Mohammad Taghdisi2؛ Khalil Abnous3؛ Mohsen Ebrahimi* 1
1Department of Pharmacology and Toxicology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
2Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3Pharmaceutical Research Center, Pharmaceutical Technology Institute, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Access to safe drinking and irrigation water has always been one of the major human concerns worldwide. Thus, rapid, sensitive, and inexpensive approaches for pathogenic bacteria detection, such as Escherichia coli O157:H7 (EHEC) that can induce important infectious diseases, are highly on demand.
Materials and Methods: In this study, a sensitive aptamer-based AuNPs bioassay was developed that demonstrated its potential to detect EHEC. In the presence of the target bacterium, the specific adsorbed aptamer, leaves AuNPs surface and interacts with EHEC. The bare nanoparticles aggregate in the presence of NaCl and the color shifts from red to purple and blue depending on the bacterial concentration.
Results: The proposed aptasensor exhibited a good linear response over a wide concentration range of 876 to 107 CFU/ml and was closely correlated with the line equation of “y=0.0094x+0.0006” (R2= 0.9861). It also showed a low detection limit (LOD) of 263 CFU/ml (Signal/Noise=3). No response was recorded in the presence of other tested bacterial strains including Listeria monocytogenes and Salmonella typhi, indicating the high selectivity of the aptasensor.
Conclusion: This biosensor may be used as a smart device to screen water reservoirs and prevents the outbreak of EHEC-related life-threatening contagious diseases.
کلیدواژه‌ها
Aptamer؛ Aptasensor؛ AuNPs؛ Escherichia coli O157:H7؛ Water
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