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Moradi, Bagher, Sankian, Mojtaba, Amini, Yousef, Gholoobi, Aida, Meshkat, Zahra. (1399). A new DNA vaccine expressing HspX-PPE44-EsxV fusion antigens of Mycobacterium tuberculosis induced strong immune responses. , 23(7), 909-914. doi: 10.22038/ijbms.2020.38521.9171
Bagher Moradi; Mojtaba Sankian; Yousef Amini; Aida Gholoobi; Zahra Meshkat. "A new DNA vaccine expressing HspX-PPE44-EsxV fusion antigens of Mycobacterium tuberculosis induced strong immune responses". , 23, 7, 1399, 909-914. doi: 10.22038/ijbms.2020.38521.9171
Moradi, Bagher, Sankian, Mojtaba, Amini, Yousef, Gholoobi, Aida, Meshkat, Zahra. (1399). 'A new DNA vaccine expressing HspX-PPE44-EsxV fusion antigens of Mycobacterium tuberculosis induced strong immune responses', , 23(7), pp. 909-914. doi: 10.22038/ijbms.2020.38521.9171
Moradi, Bagher, Sankian, Mojtaba, Amini, Yousef, Gholoobi, Aida, Meshkat, Zahra. A new DNA vaccine expressing HspX-PPE44-EsxV fusion antigens of Mycobacterium tuberculosis induced strong immune responses. , 1399; 23(7): 909-914. doi: 10.22038/ijbms.2020.38521.9171

A new DNA vaccine expressing HspX-PPE44-EsxV fusion antigens of Mycobacterium tuberculosis induced strong immune responses

Iranian Journal of Basic Medical Sciences
مقاله 11، دوره 23، شماره 7، مهر 2020، صفحه 909-914    اصل مقاله (753.09 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.38521.9171
نویسندگان
Bagher Moradi1؛ Mojtaba Sankian2؛ Yousef Amini3؛ Aida Gholoobi4؛ Zahra Meshkat* 5
1Esfarayen Faculty of Medical Sciences, Esfarayen, Iran
2Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
3Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran
4Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
5Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Infection with tuberculosis (TB) is regarded as a major health issue. Due to the emergence of antibiotic resistance during TB treatment, prevention via vaccination is one of the most effective ways of controlling the infection. DNA vaccines are developed at a greater pace due to their ability in generating a long-lasting immune response, higher safety compared to the live vaccines, and relatively lower cost of production. In the present study, we evaluated a new DNA vaccine encoding the fusion HspX-PPE44-EsxV antigens, separately, and in combination with Bacillus Calmette–Guérin (BCG) administration, in a prime-boost method in mice.
Materials and Methods: A novel DNA vaccine encoding HspX-PPE44-EsxV fusion antigen of Mycobacterium tuberculosis was constructed, and RT-PCR and Western blot analysis were performed to verify the expression of the antigen. Female BALB/c mice were divided into five groups (PBS, BCG, pcDNA3.1 (+) vector, pDNA/HspX-PPE44-EsxV vaccine, and the BCG-prime boost groups).  In order to evaluate the immunogenicity of the recombinant vector, BALB/c mice were injected with 100 μg of pDNA at 2-week intervals. Then, cytokine assay was conducted using eBioscience ELISA kits (Ebioscience, AUT) according to manufacturers’ instructions to evaluate the concentrations of IL-4, IL-12, TGF-β, and IFN-γ.
Results: The concentrations of INF-γ, IL-12, and TGF-beta were significantly increased compared to the control groups (P<0.001). INF-γ and IL-12 production were increased significantly in pDNA/HspX-PPE44-EsxV+BCG group compared to pDNA/HspX-PPE44-EsxV group (P<0.001).
Conclusion: This study showed that the present DNA vaccine could induce a high level of specific cytokines in mice. It was also shown that using this DNA vaccine in a BCG prime-boost protocol can produce significant amounts of IFN-γ, IL-12, and TGF-β.
کلیدواژه‌ها
BCG؛ DNA؛ Mycobacterium tuberculosis PCR؛ Vaccine
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