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Lei, Li, Yiqun, Pang, Zhang, Linlin, Li, Meng, Chen, Zhu, Fang, Shiyuan, Zongsheng, Yin. (1399). Triiodothyronine potentiates angiogenesis-related factor expression through PI3K/AKT signaling pathway in human osteoarthritic osteoblasts. , 23(6), 819-825. doi: 10.22038/ijbms.2020.43634.10252
Li Lei; Pang Yiqun; Linlin Zhang; Meng Li; Zhu Chen; Shiyuan Fang; Yin Zongsheng. "Triiodothyronine potentiates angiogenesis-related factor expression through PI3K/AKT signaling pathway in human osteoarthritic osteoblasts". , 23, 6, 1399, 819-825. doi: 10.22038/ijbms.2020.43634.10252
Lei, Li, Yiqun, Pang, Zhang, Linlin, Li, Meng, Chen, Zhu, Fang, Shiyuan, Zongsheng, Yin. (1399). 'Triiodothyronine potentiates angiogenesis-related factor expression through PI3K/AKT signaling pathway in human osteoarthritic osteoblasts', , 23(6), pp. 819-825. doi: 10.22038/ijbms.2020.43634.10252
Lei, Li, Yiqun, Pang, Zhang, Linlin, Li, Meng, Chen, Zhu, Fang, Shiyuan, Zongsheng, Yin. Triiodothyronine potentiates angiogenesis-related factor expression through PI3K/AKT signaling pathway in human osteoarthritic osteoblasts. , 1399; 23(6): 819-825. doi: 10.22038/ijbms.2020.43634.10252

Triiodothyronine potentiates angiogenesis-related factor expression through PI3K/AKT signaling pathway in human osteoarthritic osteoblasts

Iranian Journal of Basic Medical Sciences
مقاله 15، دوره 23، شماره 6، شهریور 2020، صفحه 819-825    اصل مقاله (990.56 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.43634.10252
نویسندگان
Li Lei1، 2؛ Pang Yiqun3؛ Linlin Zhang4؛ Meng Li4؛ Zhu Chen4؛ Shiyuan Fang4؛ Yin Zongsheng* 2
1Department of Orthopaedics,the First Affiliated Hospital of University of Science and Technology of China, #17 Lujiang Road, Hefei, Anhui, China
2Department of Orthopaedics, the First Affiliated Hospital of Anhui Medical University, #269 Jixi Road, Hefei, Anhui, China
3Department of Radiology, the First Affiliated Hospital of University of Science and Technology of China, #17 Lujiang Road, Hefei, Anhui, China
4Department of Orthopaedics,The First Affiliated Hospital of University of Science and Technology of China, #17 Lujiang Road, Hefei, Anhui, China
چکیده
Objective(s): Previous study has indicated that triiodothyronine (T3) facilitated cartilage degeneration in osteoarthritis (OA). This study aimed to investigate the effects of T3 on angiogenesis-related factor expression in human osteoblasts of OA subchondral bone.
Materials and Methods: The subchondral bone specimens were obtained from OA patients and healthy participants. The expressions of VEGF, HIF-1α, AKT, and phosphorylated AKT was detected by immunohistochemistry, Western blotting, and RT-qPCR in OA. Angiogenesis-related factor expression in OA osteoblasts was measured by treating different concentrations of T3. The hypoxia model and PX-478 (HIF-1α inhibitor) were employed to confirm the regulative role of HIF-1α for VEGF expression. The level of VEGF secretion was examined in osteoblasts supernatant using ELISA.   
Results: Immunohistochemistry showed strong staining of VEGF and HIF-1α in OA subchondral bone. The expression of VEGF, HIF-1α, and p-AKT in OA osteoblasts was higher than that of normal osteoblasts at protein and mRNA levels. The physiological concentration of T3 (10-7 M) in OA osteoblasts up-regulated the expression of VEGF, HIF-1α, and p-AKT after 24 hr and 48 hr culture, while a higher dose of T3 displayed the adverse effects. Additionally, VEGF and p-AKT expression was down-regulated when PX-478 inhibited HIF-1α protein.
Conclusion: Our results suggested that local T3 could effectively increase angiogenesis-related factor expression by PI3K/AKT signaling pathway, and HIF-1α regulated the VEGF expression in OA osteoblasts.
کلیدواژه‌ها
HIF-1α؛ Osteoarthritis؛ Osteoblast؛ PI3K؛ Thyroid hormone؛ VEGF
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