سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Vafaei, Homeira, Kavari, Ghazal, Izadi, Hamid Reza, Zare Dorahi, Zahra, Dianatpour, Mehdi, Daneshparvar, Afrooz, Jamhiri, Iman. (1399). Wi-Fi (2.4 GHz) affects anti-oxidant capacity, DNA repair genes expression and apoptosis in pregnant mouse placenta. , 23(6), 833-840. doi: 10.22038/ijbms.2020.40184.9512
Homeira Vafaei; Ghazal Kavari; Hamid Reza Izadi; Zahra Zare Dorahi; Mehdi Dianatpour; Afrooz Daneshparvar; Iman Jamhiri. "Wi-Fi (2.4 GHz) affects anti-oxidant capacity, DNA repair genes expression and apoptosis in pregnant mouse placenta". , 23, 6, 1399, 833-840. doi: 10.22038/ijbms.2020.40184.9512
Vafaei, Homeira, Kavari, Ghazal, Izadi, Hamid Reza, Zare Dorahi, Zahra, Dianatpour, Mehdi, Daneshparvar, Afrooz, Jamhiri, Iman. (1399). 'Wi-Fi (2.4 GHz) affects anti-oxidant capacity, DNA repair genes expression and apoptosis in pregnant mouse placenta', , 23(6), pp. 833-840. doi: 10.22038/ijbms.2020.40184.9512
Vafaei, Homeira, Kavari, Ghazal, Izadi, Hamid Reza, Zare Dorahi, Zahra, Dianatpour, Mehdi, Daneshparvar, Afrooz, Jamhiri, Iman. Wi-Fi (2.4 GHz) affects anti-oxidant capacity, DNA repair genes expression and apoptosis in pregnant mouse placenta. , 1399; 23(6): 833-840. doi: 10.22038/ijbms.2020.40184.9512

Wi-Fi (2.4 GHz) affects anti-oxidant capacity, DNA repair genes expression and apoptosis in pregnant mouse placenta

Iranian Journal of Basic Medical Sciences
مقاله 17، دوره 23، شماره 6، شهریور 2020، صفحه 833-840    اصل مقاله (696.8 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.40184.9512
نویسندگان
Homeira Vafaei1؛ Ghazal Kavari1؛ Hamid Reza Izadi2؛ Zahra Zare Dorahi3؛ Mehdi Dianatpour3، 4؛ Afrooz Daneshparvar3؛ Iman Jamhiri* 3
1Maternal Fetal Medicine Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4Department of Human Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Objective(s): The placenta provides nutrients and oxygen to embryo and removes waste products from embryo’s blood. As far as we know, the effects of exposure to Wi-Fi (2.4 GHz) signals on placenta have not been evaluated. Hence, we examined the effect of prenatal exposure to Wi-Fi signals on anti-oxidant capacity, expressions of CDKNA1, and GADD45a as well as apoptosis in placenta and pregnancy outcome.
Materials and Methods: Pregnant mice were exposed to Wi-Fi signal (2.4 GHz) for 2 and 4 hr. Placenta tissues were examined to measure the MDA and SOD levels. To measure SOD, CDKNA1, GADD45a, Bax, and Bcl-2 expressions were compared by real-time PCR analysis. TUNEL assay was used to assess apoptosis in placenta tissues. The results were analyzed by one-way analysis of variance (ANOVA) using Prism version 6.0 software.
Results: MDA and SOD levels had significantly increased in exposed Wi-Fi signal groups (P-value< 0.05). Also, quantitative PCR experiment showed that SOD mRNA expression significantly increased in Wi-Fi signal groups. The data showed that CDKN1A and GADD45a genes were increased in Wi-Fi groups (P-value<0.05). The quantitative PCR and the TUNEL assay showed that apoptosis increased in Wi-Fi groups (P-value<0.05).
Conclusion: Our results provide evidence that Wi-Fi signals increase lipid peroxidation, SOD activity (oxidative stres), apoptosis and CDKN1A and GADD45a overexpression in mice placenta tissue. However, further experimental studies are warranted to investigate other genes and aspects of pregnancy to determine the role of Wi-Fi radiation on fertility and pregnancy.
کلیدواژه‌ها
Anti-oxidant؛ Apoptosis؛ DNA repair؛ Placenta؛ Radiation exposure
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