سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Dalirfardouei, Razieh, Tafaghodi, Mohsen, Meshkat, zahra, Najafi, Adel, Gholoobi, Aida, Nabavinia, Maryam, Sajedifar, Samineh, Meshkat, Mojtaba, Badiee, Ali, Ramezani, Mohammad, Varasteh, Abdolreza, Naderinasab, Mahboobeh. (1399). A novel formulation of Mtb72F DNA vaccine for immunization against tuberculosis. , 23(6), 826-832. doi: 10.22038/ijbms.2020.41806.9881
Razieh Dalirfardouei; Mohsen Tafaghodi; zahra Meshkat; Adel Najafi; Aida Gholoobi; Maryam Nabavinia; Samineh Sajedifar; Mojtaba Meshkat; Ali Badiee; Mohammad Ramezani; Abdolreza Varasteh; Mahboobeh Naderinasab. "A novel formulation of Mtb72F DNA vaccine for immunization against tuberculosis". , 23, 6, 1399, 826-832. doi: 10.22038/ijbms.2020.41806.9881
Dalirfardouei, Razieh, Tafaghodi, Mohsen, Meshkat, zahra, Najafi, Adel, Gholoobi, Aida, Nabavinia, Maryam, Sajedifar, Samineh, Meshkat, Mojtaba, Badiee, Ali, Ramezani, Mohammad, Varasteh, Abdolreza, Naderinasab, Mahboobeh. (1399). 'A novel formulation of Mtb72F DNA vaccine for immunization against tuberculosis', , 23(6), pp. 826-832. doi: 10.22038/ijbms.2020.41806.9881
Dalirfardouei, Razieh, Tafaghodi, Mohsen, Meshkat, zahra, Najafi, Adel, Gholoobi, Aida, Nabavinia, Maryam, Sajedifar, Samineh, Meshkat, Mojtaba, Badiee, Ali, Ramezani, Mohammad, Varasteh, Abdolreza, Naderinasab, Mahboobeh. A novel formulation of Mtb72F DNA vaccine for immunization against tuberculosis. , 1399; 23(6): 826-832. doi: 10.22038/ijbms.2020.41806.9881

A novel formulation of Mtb72F DNA vaccine for immunization against tuberculosis

Iranian Journal of Basic Medical Sciences
مقاله 16، دوره 23، شماره 6، شهریور 2020، صفحه 826-832    اصل مقاله (679.33 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.41806.9881
نویسندگان
Razieh Dalirfardouei1، 2؛ Mohsen Tafaghodi3؛ zahra Meshkat4؛ Adel Najafi5؛ Aida Gholoobi6؛ Maryam Nabavinia7؛ Samineh Sajedifar8؛ Mojtaba Meshkat9؛ Ali Badiee3؛ Mohammad Ramezani3؛ Abdolreza Varasteh10؛ Mahboobeh Naderinasab* 4
1Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
2Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3Nanotechnology Research Center, Institute of Pharmaceutical Technology, Mashhad University of Medical Sciences, Mashhad, Iran
4Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
5Laboratory Division, Fatemieh Hospital, Hamadan University of Medical Sciences, Hamadan, Iran
6Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
7Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
8Mashhad University of Medical Sciences, Mashhad, Iran
9Mashhad Branch, Isalmic Azad University, Mashhad, Iran
10Immunobiochemistry Lab, Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Mycobacterium tuberculosis (M. tuberculosis), an intracellular pathogen, causes 1.5 million deaths globally. Bacilli Calmette-Guérin (BCG) is commonly administered to protect people against M. tuberculosis infection; however, there are some obstacles with this first-generation vaccine. DNA vaccines, the third generation vaccines, can induce cellular immune responses for tuberculosis (TB) protection. In this study, optimized DNA vaccine (pcDNA3.1-Mtb72F) entrapped in poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) was used to achieve higher immunogenicity.
Materials and Methods: Plasmid Mtb72F was formulated in PLGA NPs using double emulsion method in the presence of TB10.4 and/or CpG as an adjuvant. Female BALB/c mice were immunized either with NP-encapsulated Mtb72F or naked Mtb72F with or without each adjuvant, using the BCG-prime DNA boost regimen.
Results: These NPs were approximately 250 nm in diameter and the nucleic acid and protein encapsulation efficiency were 80% and 25%, respectively. The NPs smaller than 200 nm are able to promote cellular rather than humoral responses. The immunization with the formulation consisting of Mtb72F DNA vaccine and TB10.4 entrapped in PLGA NPs showed significant immunogenicity and induced predominantly interferon-ɣ (IFN-ɣ) production and higher INF-ɣ/interleukin-4 (IL-4) ratio in the cultured spleen cells supernatant.
Conclusion: PLGA NPs loaded with Mtb72F DNA-based vaccine with TB10.4 could be considered as a promising candidate for vaccination against TB. These results represent an excellent initial step toward development of novel vaccine for TB protection.
کلیدواژه‌ها
CpG ODN؛ DNA vaccine؛ Mtb72F؛ Mycobacterium tuberculosis؛ PLGA nanoparticles؛ Tuberculosis vaccine
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