سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Mohajer Ansari, Javad, Ramhormozi, Parisa, Shabani, Ronak, Pazoki-toroudi, Hamidreza, Yari, Abazar, Barati, Mahmood, Dahmardehei, Mostafa, Babakhani, Azar, Nobakht, Maliheh. (1399). Simvastatin combined with bone marrow mesenchymal stromal cells (BMSCs) improve burn wound healing by ameliorating angiogenesis through SDF-1α/CXCR4 pathway. , 23(6), 751-759. doi: 10.22038/ijbms.2020.39782.9465
Javad Mohajer Ansari; Parisa Ramhormozi; Ronak Shabani; Hamidreza Pazoki-toroudi; Abazar Yari; Mahmood Barati; Mostafa Dahmardehei; Azar Babakhani; Maliheh Nobakht. "Simvastatin combined with bone marrow mesenchymal stromal cells (BMSCs) improve burn wound healing by ameliorating angiogenesis through SDF-1α/CXCR4 pathway". , 23, 6, 1399, 751-759. doi: 10.22038/ijbms.2020.39782.9465
Mohajer Ansari, Javad, Ramhormozi, Parisa, Shabani, Ronak, Pazoki-toroudi, Hamidreza, Yari, Abazar, Barati, Mahmood, Dahmardehei, Mostafa, Babakhani, Azar, Nobakht, Maliheh. (1399). 'Simvastatin combined with bone marrow mesenchymal stromal cells (BMSCs) improve burn wound healing by ameliorating angiogenesis through SDF-1α/CXCR4 pathway', , 23(6), pp. 751-759. doi: 10.22038/ijbms.2020.39782.9465
Mohajer Ansari, Javad, Ramhormozi, Parisa, Shabani, Ronak, Pazoki-toroudi, Hamidreza, Yari, Abazar, Barati, Mahmood, Dahmardehei, Mostafa, Babakhani, Azar, Nobakht, Maliheh. Simvastatin combined with bone marrow mesenchymal stromal cells (BMSCs) improve burn wound healing by ameliorating angiogenesis through SDF-1α/CXCR4 pathway. , 1399; 23(6): 751-759. doi: 10.22038/ijbms.2020.39782.9465

Simvastatin combined with bone marrow mesenchymal stromal cells (BMSCs) improve burn wound healing by ameliorating angiogenesis through SDF-1α/CXCR4 pathway

Iranian Journal of Basic Medical Sciences
مقاله 7، دوره 23، شماره 6، شهریور 2020، صفحه 751-759    اصل مقاله (913.09 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.39782.9465
نویسندگان
Javad Mohajer Ansari1؛ Parisa Ramhormozi1؛ Ronak Shabani1؛ Hamidreza Pazoki-toroudi2؛ Abazar Yari3، 4؛ Mahmood Barati5؛ Mostafa Dahmardehei6؛ Azar Babakhani1؛ Maliheh Nobakht* 1، 7
1Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2Physiology research center and department of physiology, faculty of medicine, Iran University of Medical Sciences, Tehran, Iran
3Department of Anatomy, faculty of medicine, Alborz University of Medical Sciences, Karaj, Iran
4Dietary Supplements and Probiotics Research Center, Alborz University of Medical Sciences, Karaj, Iran
5Deparment of medical biotechnology, faculty of allied medicine, Iran University of Medical Sciences, Tehran, Iran
6Burn research center, Iran University of Medical Sciences, Tehran, Iran
7Anti-Microbial Resistance Research Center, Iran University of Medical Sciences, Tehran, Iran
چکیده
Objective(s): Chemokines are wound mediators that promote angiogenesis during wound healing. We hypothesized that Simvastatin in combination with the bone marrow mesenchymal stromal cells (BMSCs) improve burn wound healing by ameliorating angiogenesis via SDF-1α/CXCR4 pathway.
Materials and Methods: Under general anesthesia, deep partial-thickness burns were created on the inter-scapular area of 48 male rats. Study groups were administrated with petroleum jelly (Simvastatin Vehicle), a single dose of intradermal BMSCs (1×106), topical Simvastatin (0.5 mg/kg) daily and combination of BMSCs and Simvastatin for 14 days. In this study, we used MTT assay, in vivo and in vitro wound closure, H&E and Trichorome staining, immunohistochemistry (IHC), real- time PCR, Western blot and tube formation assay.
Results: A significant improvement in wound closure percentage, epithelial thickness, collagen remodeling, and up-regulation of stromal cell-derived factor 1 alpha (SDF1α), C-X-C chemokine receptor type 4 (CXCR4), protein kinase B (AKT), and phosphatidylinositol 3- kinase (PI3K), as well as CD31 and vascular endothelial growth factor (VEGF) expression were observed after treatment with simvastatin, BMSCs and combination of them compared to the vehicle group. However, the co-treatment group revealed considerable superiority in examined factors. BMSCs treated with Simvastatin showed the highest viability in the concentration of 0.5 and 1 Nanomolar (nM). Increment in proliferation and capillary vessels formation of BMSCs was observed in the 0.5 nM and 1 nM concentrations of Simvastatin in vitro.
Conclusion: Treatment of deep partial-thickness of burns with co-treatment of BMSCs and Simvastatin resulted in improved burn wound healing through up-regulating of SDF-1α/CXCR4 pathway.
کلیدواژه‌ها
Angiogenesis؛ Bone marrow mesenchymal stromal cells؛ C-X-C motif chemokine receptor 4؛ Stromal drive factor-1α؛ Simvastatin؛ Wound healing
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