Comparison of the effects of intramyocardial and intravenous injections of human mesenchymal stem cells on cardiac regeneration after heart failure | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 7، دوره 23، شماره 7، مهر 2020، صفحه 879-885 اصل مقاله (703.62 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2020.40886.9660 | ||
| نویسندگان | ||
| Behnaz Mokhtari1، 2؛ Nahid Aboutaleb* 1، 2؛ Donya Nazarinia1، 2؛ Mahin Nikougoftar3؛ Seyed Mohammad Taghi Razavi Tousi4؛ Mohammad Molazem5؛ Mohammad-Reza Azadi1 | ||
| 1Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran | ||
| 2Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 3Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran | ||
| 4Medical Biotechnology Research Center, Guilan University of Medical Sciences, Rasht, Iran | ||
| 5Department of Veterinary Diagnostic Imaging, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| چکیده | ||
| Objective(s): Existing studies have demonstrated that intravenous and intramyocardial-administrated mesenchymal stem cells (MSCs) lead to tissue repair after cardiac disorders. We compared the efficiency of both administration methods. Materials and Methods: A rat model of isoproterenol-induced heart failure (ISO-HF) was established to compare the effects of intravenous and intramyocardial-administrated MSCs on cardiac fibrosis and function. The animals were randomly assigned into six groups: i) control or normal, ii) ISO-HF (HF) iii) ISO-HF rats treated with intramyocardial administration of culture medium (HF+IM/CM), iv) ISO-HF rats treated with intravenous administration of culture medium ( HF+IV/CM), v) ISO-HF rats treated with intravenous administration of MSCs (HF+IV/MSCs), vi) ISO-HF rats treated with intramyocardial administration of MSCs ( HF+IM/MSCs). Cultured MSCs and culture medium were administrated at 4 weeks after final injection of ISO. Heart function, identification of MSCs, osteogenic differentiation, adipogenic differentiation, cardiac fibrosis and tissue damage were evaluated by echocardiography, flow-cytometery, von Kossa, oil red O, Masson’s trichrome and H & E staining, respectively. Results: Both intravenous and intramyocardial MSCs therapy significantly improved heart function and reduced cardiac fibrosis and tissue damage (P<0.05), whereas the cultured medium had no beneficial effects. Conclusion: In sum, our results confirm the validity of both administration methods in recovery of HF, but more future research is required. | ||
| کلیدواژهها | ||
| Heart function؛ Intramyocardial injection؛ Intravenous injection؛ Isoproterenol-induced heart failure؛ Mesenchymal stem cells | ||
| مراجع | ||
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1. Pfau D, Thorn SL, Zhang J, Mikush N, Renaud JM, Klein R, et al. Angiotensin receptor neprilysin inhibitor attenuates myocardial remodeling and improves infarct perfusion in experimental heart failure. Sci Rep 2019; 9:5791. 2. Amani H, Habibey R, Hajmiresmail S, Latifi S, Pazoki-Toroudi H, Akhavan O. Antioxidant nanomaterials in advanced diagnoses and treatments of ischemia reperfusion injuries. J Mater Chem B 2017; 5:9452-9476. 3. Saporito F, Baugh LM, Rossi S, Bonferoni MC, Perotti C, Sandri G, et al. In Situ gelling scaffolds loaded with platelet growth factors to improve cardiomyocyte survival after ischemia. ACS Biomater Sci Eng 2018; 5:329-338. 4. Li L, Zhang S, Zhang Y, Yu B, Xu Y, Guan Z. Paracrine action mediate the antifibrotic effect of transplanted mesenchymal stem cells in a rat model of global heart failure. Mol Biol Rep 2009; 36:725-731. 5. Zhang W, Zhang J, Kang Y, Liu J, Wang X, Xu Q, et al. Cardioprotective effects of oxymatrine on isoproterenol-induced heart failure via regulation of DDAH/ADMA metabolism pathway in rats. Eur J Pharmacol 2014; 745:29-35. 6. Chia N, Fulcher J, Keech A. Beta‐blocker, angiotensin‐converting enzyme inhibitor/angiotensin receptor blocker, nitrate‐hydralazine, diuretics, aldosterone antagonist, ivabradine, devices and digoxin (BANDAID2): an evidence‐based mnemonic for the treatment of systolic heart failure. Intern Med J 2016; 46:653-662. 7. Li L, Zhang Y, Li Y, Yu B, Xu Y, Zhao S, et al. Mesenchymal stem cell transplantation attenuates cardiac fibrosis associated with isoproterenol‐induced global heart failure. Transpl Int, 2008; 21:1181-1189. 8. Amani H, Mostafavi E, Arzaghi H, Davaran S, Akbarzadeh A, Akhavan O, et al. Three-dimensional graphene foams: synthesis, properties, biocompatibility, biodegradability, and applications in tissue engineering. ACS Biomater Sci Eng 2018; 5:193-214. 9. Sun S-q, Wang X-t, Qu X-f, Li Y, Yu Y, Song Y, et al. Increased expression of myocardial semaphorin 3A in isoproterenol-induced heart failure rats. Chin Med J 2011; 124:2173. 10. Arabian M, Aboutaleb N, Soleimani M, Mehrjerdi FZ, Ajami M, Pazoki-Toroudi H. Role of morphine preconditioning and nitric oxide following brain ischemia reperfusion injury in mice. Iran J Basic Med Sci 2015; 18:14-21. 11. Pazoki-Toroudi H, Nassiri-Kashani M, Tabatabaie H, Ajami M, Habibey R, Shizarpour M, et al. Combination of azelaic acid 5% and erythromycin 2% in the treatment of acne vulgaris. J Dermatol Treat 2010; 21:212-216. 12. Kumar M, Kasala ER, Bodduluru LN, Dahiya V, Lahkar M. Baicalein protects isoproterenol induced myocardial ischemic injury in male Wistar rats by mitigating oxidative stress and inflammation. Inflamm Res 2016; 65:613-622. 13. Ullah I, Subbarao RB, Rho GJ. Human mesenchymal stem cells-current trends and future prospective. Biosci Rep 2015; 35:e00191. 14. Amani H, Arzaghi H, Bayandori M, Dezfuli AS, Pazoki‐Toroudi H, Shafiee A, et al. Controlling cell behavior through the design of biomaterial surfaces: A focus on surface modification techniques. Adv Mater Interfaces 2019:1900572. 15. Premer C, Blum A, Bellio MA, Schulman IH, Hurwitz BE, Parker M, et al. Allogeneic mesenchymal stem cells restore endothelial function in heart failure by stimulating endothelial progenitor cells. EBioMedicine 2015; 2:467-475. 16. Tousi SMTR, Faghihi M, Nobakht M, Molazem M, Kalantari E, Azar AD, et al. Improvement of heart failure by human amniotic mesenchymal stromal cell transplantation in rats. J Tehran Heart Cent 2016; 11:123-138. 17. Malliaras K, Li T-S, Luthringer D, Terrovitis J, Cheng K, Chakravarty T, et al. Safety and efficacy of allogeneic cell therapy in infarcted rats transplanted with mismatched cardiosphere-derived cells. Circulation 2011; 125:100-112. 18. Gnecchi M, Zhang Z, Ni A, Dzau VJ. Paracrine mechanisms in adult stem cell signaling and therapy. Circ Res 2008; 103:1204-1219. 19. Li Y, Zhang Y, Li Z, Zhou K, Feng N. Exosomes as Carriers for Antitumor Therapy. ACS Biomater Sci Eng 2019; 5, 10, 4870-4881. 20. Nazarinia D, Aboutaleb N, Gholamzadeh R, Maleki SN, Mokhtari B, Nikougoftar M. Conditioned medium obtained from human amniotic mesenchymal stem cells attenuates focal cerebral ischemia/reperfusion injury in rats by targeting mTOR pathway. J Chem Neuroanat 2019; 102:101707. 21. Amirfarhangi A, Dezfouli M, Abarkar M, Rakhshan K, Aboutaleb N. Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected Cytokines. Int J Stem Cell Res Transplant 2016; 4:243-250. 22. Lee S-T, White AJ, Matsushita S, Malliaras K, Steenbergen C, Zhang Y, et al. Intramyocardial injection of autologous cardiospheres or cardiosphere-derived cells preserves function and minimizes adverse ventricular remodeling in pigs with heart failure post-myocardial infarction. J Am Coll Cardiol 2011; 57:455-465. 23. Amado LC, Saliaris AP, Schuleri KH, John MS, Xie J-S, Cattaneo S, et al. Cardiac repair with intramyocardial injection of allogeneic mesenchymal stem cells after myocardial infarction. Proc Natl Acad Sci 2005; 102:11474-11479. 24. Luger D, Lipinski MJ, Westman PC, Glover DK, Frias JC, Albelda M, et al. Intravenously-delivered mesenchymal stem cells: systemic anti-inflammatory effects improve left ventricular dysfunction in acute myocardial infarction and ischemic cardiomyopathy. Circ Res 2017; 102: 11474-11479. 25. Faezi M, Maleki SN, Aboutaleb N, Nikougoftar M. The membrane mesenchymal stem cell derived conditioned medium exerts neuroprotection against focal cerebral ischemia by targeting apoptosis J Chem Neuroanat 2018; 94:21-31. 26. Aboutaleb N, Faezi M, Maleki SN, Nazarinia D, Tousi SMTR, Hashemirad N. Conditioned medium obtained from mesenchymal stem cells attenuates focal cerebral ischemia reperfusion injury through activation of ERK1/ERK2-BDNF signaling pathway. J Chem Neuroanat 2019; 97:87-98. 27. Nasseri Maleki S, Aboutaleb N, Nazarinia D, Allahverdi Beik S, Qolamian A, Nobakht M. Conditioned medium obtained from human amniotic membrane-derived mesenchymal stem cell attenuates heart failure injury in rats. Iran J Basic Med Sci 2019; 22:1253-1258. 28. Meeran MFN, Jagadeesh GS, Selvaraj P. Catecholamine toxicity triggers myocardial membrane destabilization in rats: thymol and its counter action. RSC Adv. 2015; 5:43338-43344. 29. Tousi SMTR, Amirizadeh N, Nasirinezhad F, Nikougoftar M, Ganjibakhsh M, Aboutaleb N. A rapid and cost-effective protocol for isolating mesenchymal stem cells from the human amniotic membrane. Galen Med J 2017; 6:217-225. 30. Li X, Yu X, Lin Q, Deng C, Shan Z, Yang M, et al. Bone marrow mesenchymal stem cells differentiate into functional cardiac phenotypes by cardiac microenvironment. J Mol Cell Cardiol 2007; 42:295-303. 31. Li W, Gan R, Sun G. Chronic treatment of enbrel in rats with isoproterenol-induced congestive heart failure limits left ventricular dysfunction and remodeling. Chin Med J 2002; 115:1166-1169. 32. Rodriguez AJ, Mousa A, Ebeling PR, Scott D, De Courten B. Effects of vitamin D supplementation on inflammatory markers in heart failure: A systematic review and meta-analysis of randomized controlled trials. Sci Rep 2018; 8:1169. 33. Amani H, Mostafavi E, Alebouyeh MR, Arzaghi H, Akbarzadeh A, Pazoki-Toroudi H, et al. Would Colloidal Gold Nanocarriers Present An Effective Diagnosis Or Treatment For Ischemic Stroke? Int J Nanomed 2019; 14:8013. 34. Amani H, Habibey R, Shokri F, Hajmiresmail SJ, Akhavan O, Mashaghi A, et al. Selenium nanoparticles for targeted stroke therapy through modulation of inflammatory and metabolic signaling. Sci Rep 2019; 9:6044. 35. Amani H, Kazerooni H, Hassanpoor H, Akbarzadeh A, Pazoki-Toroudi H. Tailoring synthetic polymeric biomaterials towards nerve tissue engineering: a review. Artif Cells Nanomed Biotechnol 2019; 47:3524-3539. 36. Javedan G, Shidfar F, Davoodi SH, Ajami M, Gorjipour F, Sureda A, et al. Conjugated linoleic acid rat pretreatment reduces renal damage in ischemia/reperfusion injury: Unraveling antiapoptotic mechanisms and regulation of phosphorylated mammalian target of rapamycin. Mol Nutr Food Res 2016; 60:2665-2677. 37. Firooz A, Bouzari N, Mojtahed F, Pazoki-Toroudi H, Nassiri-Kashani M, Davoudi M, et al. Topical immunotherapy with diphencyprone in the treatment of extensive and/or long-lasting alopecia areata 11. J Eur Acad Dermatol Venereol 2005; 19:393-394. 38. Booth EA, Lucchesi BR. Estrogen-mediated protection in myocardial ischemia-reperfusion injury. Cardiovasc Toxicol 2008; 8:101-113. 39. Cheng G, Labedz-Maslowska A, Berdecka D, Xuan Y-T, Samanta A, Girgis M, et al. Intramyocardial Injection of MSC-derived Extracellular Vesicles Confers Superior Cardiac Repair After a Reperfused Myocardial Infarction. Circulation 2015; 132:A16673. 40. Jiang Q, Yu T, Huang K, Zhang H, Zheng Z, Hu S. Systemic redistribution of the intramyocardially injected mesenchymal stem cells by repeated remote ischaemic post‐conditioning. J Cell Mol Med 2018; 22:417-428. 41. Wang W, Jin P, Wang L, Yang Z, Hu S, Gao B, et al. Impact of escaped bone marrow mesenchymal stromal cells on extracardiac organs after intramyocardial implantation in a rat myocardial infarction model. Cell Transplant 2010; 19:1599-1607. 42. Williams AR, Hatzistergos KE, Addicott B, McCall F, Carvalho D, Suncion V, et al. Enhanced effect of human cardiac stem cells and bone marrow mesenchymal stem cells to reduce infarct size and restore cardiac function after myocardial infarction. Circulation 2012; 112.131110. 43. Nagaya N, Fujii T, Iwase T, Ohgushi H, Itoh T, Uematsu M, et al. Intravenous administration of mesenchymal stem cells improves cardiac function in rats with acute myocardial infarction through angiogenesis and myogenesis. Am J Phys Heart Circ Phys 2004; 287:H2670-H2676. 44. Lim M, Wang W, Liang L, Han Z-b, Li Z, Geng J, et al. Intravenous injection of allogeneic umbilical cord-derived multipotent mesenchymal stromal cells reduces the infarct area and ameliorates cardiac function in a porcine model of acute myocardial infarction. Stem Cell Res Ther 2018; 9:129. | ||
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