سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Seyyed Tabaei, Seyyed Javad, Rahimi, Mohsen, Akbaribazm, Mohsen, Ziai, Seyed Ali, Sadri, Minoo, Shahrokhi, Seyed Reza, Rezaei, Mitra Sadat. (1399). Chitosan-based nano-scaffolds as antileishmanial wound dressing in BALB/c mice treatment: Characterization and design of tissue regeneration. , 23(6), 788-799. doi: 10.22038/ijbms.2020.41361.9770
Seyyed Javad Seyyed Tabaei; Mohsen Rahimi; Mohsen Akbaribazm; Seyed Ali Ziai; Minoo Sadri; Seyed Reza Shahrokhi; Mitra Sadat Rezaei. "Chitosan-based nano-scaffolds as antileishmanial wound dressing in BALB/c mice treatment: Characterization and design of tissue regeneration". , 23, 6, 1399, 788-799. doi: 10.22038/ijbms.2020.41361.9770
Seyyed Tabaei, Seyyed Javad, Rahimi, Mohsen, Akbaribazm, Mohsen, Ziai, Seyed Ali, Sadri, Minoo, Shahrokhi, Seyed Reza, Rezaei, Mitra Sadat. (1399). 'Chitosan-based nano-scaffolds as antileishmanial wound dressing in BALB/c mice treatment: Characterization and design of tissue regeneration', , 23(6), pp. 788-799. doi: 10.22038/ijbms.2020.41361.9770
Seyyed Tabaei, Seyyed Javad, Rahimi, Mohsen, Akbaribazm, Mohsen, Ziai, Seyed Ali, Sadri, Minoo, Shahrokhi, Seyed Reza, Rezaei, Mitra Sadat. Chitosan-based nano-scaffolds as antileishmanial wound dressing in BALB/c mice treatment: Characterization and design of tissue regeneration. , 1399; 23(6): 788-799. doi: 10.22038/ijbms.2020.41361.9770

Chitosan-based nano-scaffolds as antileishmanial wound dressing in BALB/c mice treatment: Characterization and design of tissue regeneration

Iranian Journal of Basic Medical Sciences
مقاله 12، دوره 23، شماره 6، شهریور 2020، صفحه 788-799    اصل مقاله (1.19 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.41361.9770
نویسندگان
Seyyed Javad Seyyed Tabaei1؛ Mohsen Rahimi* 2؛ Mohsen Akbaribazm3؛ Seyed Ali Ziai4؛ Minoo Sadri5؛ Seyed Reza Shahrokhi6؛ Mitra Sadat Rezaei7، 8
1Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2Department of Parasitology and Mycology, School of Medicine, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Anatomical Sciences, Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
4Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5Department of Biochemistry and Biophysics, Education and Research Center of Science and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran
6Doctor of Veterinary Medicine (D.V.M.), Rasht, Iran
7Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
8Pathology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
چکیده
Objective(s): Rapid healing of cutaneous leishmaniasis as one of the most important parasitic diseases leads to the decrease of scars and prevention of a great threat to the looks of the affected people. Today, the use of nano-scaffolds is rapidly increasing in tissue engineering and regenerative medicine with structures similar to the target tissue. Chitosan (CS) is a bioactive polymer with antimicrobial and accelerating features of healing wounds, which is commonly used in biomedicine. This study aimed to investigate the effects of CS/polyethylene oxide (PEO)/berberine (BBR) nanofibers on the experimental ulcers of Leishmania major in BALB/c mice.
Materials and Methods: CS/PEO/BBR nanofibers were prepared by the electrospinning method, and their morphology was examined by SEM, TEM, and AFM. Then, water absorption, stability, biocompatibility, porosity, and drug release from nano-scaffolds were explored. Afterward, 28 BALB/c mice infected with the parasite were randomly divided into control and experimental groups, and their wounds were dressed with the produced nano-scaffolds. Finally, the effect of nanobandage on the animals was investigated by macroscopic, histopathologic, and in vivo imaging examinations.
Results: The prepared nanofibers were completely uniform, cylindrical, bead-free, and biocompatible with an average diameter of 94±12 nm and had appropriate drug release. In addition, the reduced skin ulcer diameter (P=0.000), parasite burden (P=0.003), changes in the epidermis (P=0.023), and dermis (P=0.032) indicated significantly strong effectiveness of the produced nano-scaffolds against leishmania ulcers.
Conclusion: Studies showed that CS/PEO/BBR nanofibers have a positive effect on the rapid healing of leishmania ulcers. Future studies should focus on other chronic ulcers treatment.
کلیدواژه‌ها
Bandages؛ Berberine؛ Drug-delivery system؛ Nanostructures؛ Wound healing
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