Complete ablation of tumor necrosis factor decreases the production of IgA, IgG, and IgM in experimental central nervous system tuberculosis | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 16، دوره 23، شماره 5، مرداد 2020، صفحه 680-690 اصل مقاله (1.56 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2020.37947.9021 | ||
| نویسندگان | ||
| Ngiambudulu Francisco* 1، 2، 3؛ Nasiema Allie4؛ Boipelo Sebesho5؛ Bernhard Ryffel6؛ Muazzam Jacobs5، 7، 8 | ||
| 1Program of Infection and Immunity, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhongshan School of Medicine, Sun Yat-sen University, Guangdong, China | ||
| 2Institute of Tuberculosis Control, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China | ||
| 3Key Laboratory of Tropical Diseases Control, Ministry of Education, Sun Yat-sen University, Guangzhou, China | ||
| 4SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Stellenbosch University, Stellenbosch, South Africa | ||
| 5Division of Immunology, Department of Pathology and Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town 7925, South Africa | ||
| 6Institut de Transgenose, CNRS, GEM2358, Orleans, France, University of Orleans and CNRS UMR7355, Experimental and Molecular Immunology and Neurogenetics, Orleans, France | ||
| 7National Health Laboratory Service, Sandringham, Johannesburg, South Africa | ||
| 8Immunology of Infectious Disease Research Unit, South African Medical Research Council, Cape Town 8000, South Africa | ||
| چکیده | ||
| Objective(s): This study aimed to explore the contribution of tumor necrosis factor (TNF) in the recruitment of B-cell and secretion of immunoglobulins (Igs) during cerebral tuberculosis (TB). Materials and Methods: In this work, the contributing role of TNF in regulating Ig secretions was investigated by comparing wild type TNF (TNFf/f), B-cell-derived TNF (BTNF-/-), and complete TNF ablation (TNF-/-) in a mouse cerebral Mycobacterium tuberculosis infection. Using flow cytometry and ELISA, we were able to examine the recruitment of B-cell subsets, and the production of Igs; also assessed the expression of surface markers on B cell subsets. Results: Here, we found that TNF-/- mice showed defective expression of IgA, IgG, and IgM antibodies compared with TNFf/f and BTNF-/- mice, which was significantly decreased in the expression of surface markers and co-stimulatory molecules. Moreover, mice that produced low antibody levels were not able to control infection, therefore progressed to disease; providing direct evidence for the TNF gene-regulating humoral immunity during central nervous system (CNS) M. tuberculosis infection. In contrast, BTNF-/- mice controlled the infection and had levels of IgA, IgG, and IgM comparable to TNFf/f mice. Conclusion: Together, our results demonstrate that TNF may serve as an essential regulator of antibody-mediated immune responses in CNS TB. However, the protective level exhibited by TNF-producing B cells could be defined as baseline protection that could be used in the development of new therapeutic targets or designing new vaccines. | ||
| کلیدواژهها | ||
| Antibody؛ Central nervous system؛ Humoral؛ Immunity infections؛ Mycobacterium؛ Tumor necrosis factor | ||
| مراجع | ||
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