سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Ghasemi, Ali, Falak, Reza, Mohamadi, Mohsen, June Maleki, Soheila, Assarezadegan, Mohammad-Ali, Jafary, Mojtaba. (1399). Incorporation of T-cell epitopes from tetanus and diphtheria toxoids into in-silico-designed hypoallergenic vaccine may enhance the protective immune response against allergens. , 23(5), 636-644. doi: 10.22038/ijbms.2020.39749.9422
Ali Ghasemi; Reza Falak; Mohsen Mohamadi; Soheila June Maleki; Mohammad-Ali Assarezadegan; Mojtaba Jafary. "Incorporation of T-cell epitopes from tetanus and diphtheria toxoids into in-silico-designed hypoallergenic vaccine may enhance the protective immune response against allergens". , 23, 5, 1399, 636-644. doi: 10.22038/ijbms.2020.39749.9422
Ghasemi, Ali, Falak, Reza, Mohamadi, Mohsen, June Maleki, Soheila, Assarezadegan, Mohammad-Ali, Jafary, Mojtaba. (1399). 'Incorporation of T-cell epitopes from tetanus and diphtheria toxoids into in-silico-designed hypoallergenic vaccine may enhance the protective immune response against allergens', , 23(5), pp. 636-644. doi: 10.22038/ijbms.2020.39749.9422
Ghasemi, Ali, Falak, Reza, Mohamadi, Mohsen, June Maleki, Soheila, Assarezadegan, Mohammad-Ali, Jafary, Mojtaba. Incorporation of T-cell epitopes from tetanus and diphtheria toxoids into in-silico-designed hypoallergenic vaccine may enhance the protective immune response against allergens. , 1399; 23(5): 636-644. doi: 10.22038/ijbms.2020.39749.9422

Incorporation of T-cell epitopes from tetanus and diphtheria toxoids into in-silico-designed hypoallergenic vaccine may enhance the protective immune response against allergens

Iranian Journal of Basic Medical Sciences
مقاله 11، دوره 23، شماره 5، مرداد 2020، صفحه 636-644    اصل مقاله (1.12 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.39749.9422
نویسندگان
Ali Ghasemi1؛ Reza Falak2؛ Mohsen Mohamadi* 3؛ Soheila June Maleki4؛ Mohammad-Ali Assarezadegan2؛ Mojtaba Jafary3
1Department of Biochemistry and Hematology, Faculty of Medicine Semnan University of Medical Sciences, Semnan, Iran
2Immunology Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
3The Persian Gulf Marine Biotechnology Research Center, the Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
4US Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, Louisiana, USA
چکیده
Objective(s): New generation of allergy vaccines is capable of promoting the development of protective IgG and blocking the functionality of allergen-specific IgE. We incorporated universal and powerful T-cell epitopes from tetanus and diphtheria toxoids (TD epitope) into recombinant Che a 2, the well-known allergic profilin of Chenopodium album, to determine its immunological properties.
Materials and Methods: The sequence and accordingly the structure of the recombinant Che a 2 was altered to generate a hypoallergenic variant (rChe a 2.rs). Moreover, TD epitope was incorporated to produce a novel vaccine that was nominated as rChe a 2.rsT.D. The effect of treatment with these variants was evaluated on the generation of allergen-specific IgG class, as well as lymphocyte proliferation in mice. Moreover, IgE-binding characteristics of the allergic patients’ sera were determined by ELISA and proliferation and cytokine production was measured in T-cells.
Results: ELISA and dot blot revealed strong reduction of the IgE-reactivity of human sera to the variants of Che a 2 as compared to the wild-type molecule. Furthermore, Che a 2.rs and Che a 2.rsT.D induced much lower levels of IL5 and IL13 secretion from allergic patients’ PBMCs in comparison to wild-type Che a 2 protein. In mice, rChe a 2.rsT.D induced high titers of Che a 2-specific IgG antibody capable of blocking IgE-binding to rChe a 2 and induced lymphocyte proliferation more potently than rChe a 2.rs.
Conclusion: Collectively, incorporation of T-cell epitopes of tetanus and diphtheria into hypoallergenic vaccines can dramatically enhance anti-allergic immune mechanisms, particularly in poor responders.
کلیدواژه‌ها
Allergen؛ Chenopodium album؛ Diphtheria-tetanus vaccine؛ Epitope؛ T lymphocyte
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