Nos2 deficiency enhances carbon tetrachloride-induced liver injury in aged mice

Li, Deming; Song, Yaping; Wang, Yahao; Guo, Yuedong; Zhang, Zhaoke; Yang, Ganggang; Wang, Gaiping; Xu, Cunshuan

doi:10.22038/ijbms.2020.39528.9380

	Nos2 deficiency enhances carbon tetrachloride-induced liver injury in aged mice
Iranian Journal of Basic Medical Sciences
مقاله 6، دوره 23، شماره 5، مرداد 2020، صفحه 600-605 اصل مقاله (592.26 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.39528.9380
نویسندگان
Deming Li¹^{، 2}^{، 3}^{، 4}^{، 5}^{، 6}؛ Yaping Song¹^{، 2}^{، 3}^{، 4}^{، 5}^{، 6}؛ Yahao Wang¹^{، 2}^{، 3}^{، 4}^{، 5}^{، 6}؛ Yuedong Guo¹^{، 2}^{، 3}^{، 4}^{، 5}^{، 6}؛ Zhaoke Zhang¹^{، 2}^{، 3}^{، 4}^{، 5}^{، 6}؛ Ganggang Yang¹^{، 2}^{، 3}^{، 4}^{، 5}^{، 6}؛ Gaiping Wang¹^{، 2}^{، 3}^{، 4}^{، 5}^{، 6}؛ Cunshuan Xu^* ¹^{، 2}^{، 3}^{، 4}^{، 5}^{، 6}
¹State Key Laboratory Cell Differentiation and Regulation
²Henan International Joint Laboratory of Pulmonary Fibrosis
³Henan center for outstanding overseas scientists of pulmonary fibrosis
⁴College of Life Science
⁵Institute of Biomedical Science
⁶Overseas Expertise Introduction Center for Discipline Innovation of Pulmonary Fibrosis (111 Project), Henan Normal University, Xinxiang, Henan, China
چکیده
Objective(s): As a multifunctional molecule, NO has different effects on liver injury. The present work aimed to investigate the effects of Nos2 knockout (KO) on acute liver injury in aged mice treated with carbon tetrachloride (CCl4). Materials and Methods: The acute liver injury model was produced by CCl4 at 10 ml/kg body weight in 24-month-old Nos2 KO mice and wild type (WT) mice groups. The histological changes, transaminase and glutathione (GSH) contents, and the expressions of liver function genes superoxide dismutase (SOD2) and butyrylcholinesterase (BCHE), as well as apoptosis- and inflammation-associated genes were detected at 0, 6, 16, 20, 28, and 48 hr, respectively. Results: Compared with WT aged mice, there are more fat droplets in liver tissues of Nos2 KO aged mice, and the serum levels of ALT and AST were elevated in the KO group; in addition, there was a decrease in the expression of SOD2 and BCHE and GSH content at multiple time-points. Furthermore, the expression of apoptosis protein CASPASE-3 was elevated from 20 to 48 hr, the same as CASPASE-9 at 28 and 48 hr and pro-apoptotic protein BAX at 6 and 28 hr, while the expression of apoptosis inhibitory protein BCL2 declined at 6 and 28 hr; at the same time the mRNA expressions of genes related to inflammation were increased at different extents in liver extracts of Nos2 KO aged mice. Conclusion: Nos2 KO exacerbated liver injury probably by elevated oxidative stress, apoptosis and inﬂammation response in CCl4-induced aged mice liver intoxication model.
کلیدواژه‌ها
Aged؛ CCl4؛ Knockout؛ Liver injury؛ Nos2؛ Oxidative stress
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