سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Bagheri, Morteza, Saboory, Ehsan, Nejatbakhsh, Mehrdad, Roshan-Milani, Shiva, Derafshpour, Leila, Sayyadi, Hojjat, Rasmi, Yousef. (1399). Prenatal stress increased γ2 GABAA receptor subunit gene expression in hippocampus and potentiated pentylenetetrazol-induced seizure in rats. , 23(6), 724-729. doi: 10.22038/ijbms.2020.39519.9371
Morteza Bagheri; Ehsan Saboory; Mehrdad Nejatbakhsh; Shiva Roshan-Milani; Leila Derafshpour; Hojjat Sayyadi; Yousef Rasmi. "Prenatal stress increased γ2 GABAA receptor subunit gene expression in hippocampus and potentiated pentylenetetrazol-induced seizure in rats". , 23, 6, 1399, 724-729. doi: 10.22038/ijbms.2020.39519.9371
Bagheri, Morteza, Saboory, Ehsan, Nejatbakhsh, Mehrdad, Roshan-Milani, Shiva, Derafshpour, Leila, Sayyadi, Hojjat, Rasmi, Yousef. (1399). 'Prenatal stress increased γ2 GABAA receptor subunit gene expression in hippocampus and potentiated pentylenetetrazol-induced seizure in rats', , 23(6), pp. 724-729. doi: 10.22038/ijbms.2020.39519.9371
Bagheri, Morteza, Saboory, Ehsan, Nejatbakhsh, Mehrdad, Roshan-Milani, Shiva, Derafshpour, Leila, Sayyadi, Hojjat, Rasmi, Yousef. Prenatal stress increased γ2 GABAA receptor subunit gene expression in hippocampus and potentiated pentylenetetrazol-induced seizure in rats. , 1399; 23(6): 724-729. doi: 10.22038/ijbms.2020.39519.9371

Prenatal stress increased γ2 GABAA receptor subunit gene expression in hippocampus and potentiated pentylenetetrazol-induced seizure in rats

Iranian Journal of Basic Medical Sciences
مقاله 3، دوره 23، شماره 6، شهریور 2020، صفحه 724-729    اصل مقاله (575.14 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.39519.9371
نویسندگان
Morteza Bagheri1؛ Ehsan Saboory* 2؛ Mehrdad Nejatbakhsh3؛ Shiva Roshan-Milani3، 4؛ Leila Derafshpour3، 4؛ Hojjat Sayyadi5؛ Yousef Rasmi1
1Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran
2Zanjan Metabolic Diseases Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
3Department of Physiology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
4Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran
5Department of Biostatistics , School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
چکیده
Objective(s): Stress during pregnancy is able to bring extensive effects on neurobehavioral development in offspring. The GABAergic system plays a pivotal role in neuronal excitability, which can be affected by prenatal stress (PS). This study aimed to evaluate impact of the PS on γ2 subunit of gamma-aminobutyric acid A (GABAA) receptor gene expression in the hippocampus and seizure induced by pentylenetetrazol (PTZ) in developing rats.
Materials and Methods: In this experimental study, female Wistar rats were exposed to restraint stress during gestation and their offspring were studied on postnatal days 14 and 21 (P14 and P21, respectively) for epileptic behaviors and γ2 GABAA receptor subunit gene expression. Quantitative real-time PCR was used for evaluating the γ2 GABAA receptor subunit gene expression in rat pups. Meanwhile, PTZ was injected into the pups, and seizure behaviors were recorded for 60 min.
Results: The results showed that γ2 subunit mRNA expression significantly increased in the hippocampus of the stressed pups. The expression level of γ2 subunit was higher on P21 compared to that on P14 in both groups. Number of seizures with tonic–clonic features increased in pups of stressed group compared to the control group. Prenatal stress significantly caused an increase in the total score of seizure on P21.
Conclusion: The effect of PS on seizure susceptibility is age-specific; the increased γ2 subunit level in the hippocampus might be, at least in part, the underlying mechanism for PS-induced augmentation of seizures in immature rats.
کلیدواژه‌ها
GABAA receptor؛ Infant؛ Prenatal stress؛ Rat؛ Seizure
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