سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Kadkhoda, Hiva, Ghalavand, Zohreh, Nikmanesh, Bahram, Kodori, Mansoor, Houri, Hamidreza, Taghizadeh Maleki, Donya, Karimi Bavandpour, Ali, Eslami, Gita. (1399). Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran. , 23(5), 691-698. doi: 10.22038/ijbms.2020.36299.8644
Hiva Kadkhoda; Zohreh Ghalavand; Bahram Nikmanesh; Mansoor Kodori; Hamidreza Houri; Donya Taghizadeh Maleki; Ali Karimi Bavandpour; Gita Eslami. "Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran". , 23, 5, 1399, 691-698. doi: 10.22038/ijbms.2020.36299.8644
Kadkhoda, Hiva, Ghalavand, Zohreh, Nikmanesh, Bahram, Kodori, Mansoor, Houri, Hamidreza, Taghizadeh Maleki, Donya, Karimi Bavandpour, Ali, Eslami, Gita. (1399). 'Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran', , 23(5), pp. 691-698. doi: 10.22038/ijbms.2020.36299.8644
Kadkhoda, Hiva, Ghalavand, Zohreh, Nikmanesh, Bahram, Kodori, Mansoor, Houri, Hamidreza, Taghizadeh Maleki, Donya, Karimi Bavandpour, Ali, Eslami, Gita. Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran. , 1399; 23(5): 691-698. doi: 10.22038/ijbms.2020.36299.8644

Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran

Iranian Journal of Basic Medical Sciences
مقاله 17، دوره 23، شماره 5، مرداد 2020، صفحه 691-698    اصل مقاله (603 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.36299.8644
نویسندگان
Hiva Kadkhoda1؛ Zohreh Ghalavand1؛ Bahram Nikmanesh2؛ Mansoor Kodori1؛ Hamidreza Houri1؛ Donya Taghizadeh Maleki1؛ Ali Karimi Bavandpour3؛ Gita Eslami* 1
1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
3Department of Bacteriology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran
چکیده
Objective(s): Staphylococcus aureus can cause several infections. Its capability to form biofilm has been reported to be a vital property involved in the bacteria’s pathogenesis. Various genes contributing to biofilm formation have not yet been completely clarified. This study was designed to evaluate the factors influencing adherence and biofilm formation in S. aureus isolated from paediatric patients.
Materials and Methods: One hundred and ninety-seven S. aureus isolates were obtained from pediatric patients and confirmed with phenotypic and molecular examinations. Antimicrobial susceptibility testing and biofilm formation were evaluated using standard methods. The genes encoding adhesion and virulence factors were investigated by the PCR method.
Results: The most efficient antibiotics against S. aureus isolates were vancomycin and linezolid. Approximately, 54.2% of MSSA and 85.6% of MRSA isolates were biofilm producers according to the microtiter test. Our analysis indicated that MRSA isolates are better able to form biofilm compared with MSSA isolates. All isolates harbored clfA, fnbpA, icaA, icaB, icaC, and icaD, while clfB, fnbB, hlg, and pvl were detected in 99.5%, 42.1%, 97.5%, and 5.6% of isolates, respectively. In addition, a significant difference was found in fnhB gene and biofilm formation.
Conclusion: Our findings showed a significant correlation between mecA and pvl genes and MRSA and biofilm formation in S. aureus isolates. Additionally, this study indicated the significant role of the fnhB gene as a major marker for S. aureus biofilm formation. Therefore, further experiments are warranted to exactly elucidate the function of the fnhB gene in the formation of biofilm.
کلیدواژه‌ها
Biofilm؛ Fibronectin binding-proteins؛ MRSA؛ MSCRAMMs؛ Pediatric؛ Staphylococcus aureus؛ Virulence factors
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