Hydrogen-rich saline ameliorates hippocampal neuron apoptosis through up-regulating the expression of cystathionine β-synthase (CBS) after cerebral ischemia- reperfusion in rats | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 10، دوره 23، شماره 4، تیر 2020، صفحه 494-499 اصل مقاله (788.5 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2020.41751.9857 | ||
| نویسندگان | ||
| Haiming Cong1؛ Qiu-ping Gao2؛ Guo-qiang Song3؛ Yin-gxin Ye4؛ Xiao-li Li5؛ Lian-shuang Zhang6؛ Xi-feng Wang* 5 | ||
| 1Department of Rehabilitation Medicine, Weihai Central Hospital, Wendeng 264400, China | ||
| 2Department of Emergency, Ji Nan Zhang Qiu District Hospital of Traditional Chinese Medicine, Zhang Qiu 250200, China | ||
| 3Department of Neurosurgery, Ji Nan Zhang Qiu District Hospital of Traditional Chinese Medicine, Zhang Qiu 250200, China | ||
| 4Department of Neurology, Ji Nan Zhang Qiu District Hospital of TraditionalChinese Medicine, Zhang Qiu 250200, China | ||
| 5Department of Critical Care Medicine, Yu Huang Ding Hospital, Qingdao University, Yantai, P. R. China | ||
| 6Department of Histology and Embryology, Binzhou Medical University, Yantai, P. R. China | ||
| چکیده | ||
| Objective(s): This study aimed to evaluate the potential role of hydrogen in rats after cerebral ischemic/reperfusion (I/R) injury. Materials and Methods: The experimental samples were composed of sham group, model group of rats that received middle cerebral artery occlusion (MCAO) for 2 hr followed by reperfusion for 24 hr, and the hydrogen saline group treated by hydrogen-rich saline (1 ml/kg) after MCAO. Hydrogen sulfide (H2S), S100-βprotein (S100-β), and neuron-specific enolase (NSE) levels were measured; the levels of malondialdehyde (MDA), reactive oxygen species (ROS), and superoxide dismutase (SOD) were detected; the histologic structure and apoptotic cells of hippocampus were observed; the expressions of cystathionine β-synthase (CBS), nuclear factor erythroid 2-related factor 2 (Nrf2), and hemeoxygenase-1 (HO-1) were measured. Statistical analyses were performed using one-way analysis of variance (ANOVA) followed by Fisher’s least significant difference (LSD) test. Results: Our results showed that hydrogen up-regulated H2S levels via promoting the expression of CBS in the hippocampus, and its treatment alleviated oxidative stress via activating the expression of Nrf2 and HO-1, and then cell apoptosis reduced, furthermore, brain function improved by down-regulating the levels of S100-βand NSE. Conclusion: This study showed that hydrogen-rich saline ameliorates cell injury through up-regulating the expression of CBS in the hippocampus after cerebral ischemia reperfusion (I/R) in rats, this provides new experimental evidence for the treatment of stroke with hydrogen saline. | ||
| کلیدواژهها | ||
| Cerebral ischemia reperfusion؛ Cystathionine β-synthase؛ Hippocampus؛ Hydrogen؛ Rats | ||
| مراجع | ||
|
| ||
|
آمار تعداد مشاهده مقاله: 1,153 تعداد دریافت فایل اصل مقاله: 782 |
||