سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Bahaloo, Maryam, Rezvani, Mohammad Ebrahim, Farashahi Yazd, Ehsan, Zare Mehrjerdi, Fatemeh, Davari, Mohammad Hossein, Roohbakhsh, Ali, Mollasadeghi, Abolfazl, Nikkhah, Haniyeh, Vafaei, Maryam, Mehrparvar, Amir Houshang. (1399). Effect of myricetin on the gene expressions of NOX3, TGF-β1, prestin, and HSP-70 and anti-oxidant activity in the cochlea of noise-exposed rats. , 23(5), 594-599. doi: 10.22038/ijbms.2020.41007.9693
Maryam Bahaloo; Mohammad Ebrahim Rezvani; Ehsan Farashahi Yazd; Fatemeh Zare Mehrjerdi; Mohammad Hossein Davari; Ali Roohbakhsh; Abolfazl Mollasadeghi; Haniyeh Nikkhah; Maryam Vafaei; Amir Houshang Mehrparvar. "Effect of myricetin on the gene expressions of NOX3, TGF-β1, prestin, and HSP-70 and anti-oxidant activity in the cochlea of noise-exposed rats". , 23, 5, 1399, 594-599. doi: 10.22038/ijbms.2020.41007.9693
Bahaloo, Maryam, Rezvani, Mohammad Ebrahim, Farashahi Yazd, Ehsan, Zare Mehrjerdi, Fatemeh, Davari, Mohammad Hossein, Roohbakhsh, Ali, Mollasadeghi, Abolfazl, Nikkhah, Haniyeh, Vafaei, Maryam, Mehrparvar, Amir Houshang. (1399). 'Effect of myricetin on the gene expressions of NOX3, TGF-β1, prestin, and HSP-70 and anti-oxidant activity in the cochlea of noise-exposed rats', , 23(5), pp. 594-599. doi: 10.22038/ijbms.2020.41007.9693
Bahaloo, Maryam, Rezvani, Mohammad Ebrahim, Farashahi Yazd, Ehsan, Zare Mehrjerdi, Fatemeh, Davari, Mohammad Hossein, Roohbakhsh, Ali, Mollasadeghi, Abolfazl, Nikkhah, Haniyeh, Vafaei, Maryam, Mehrparvar, Amir Houshang. Effect of myricetin on the gene expressions of NOX3, TGF-β1, prestin, and HSP-70 and anti-oxidant activity in the cochlea of noise-exposed rats. , 1399; 23(5): 594-599. doi: 10.22038/ijbms.2020.41007.9693

Effect of myricetin on the gene expressions of NOX3, TGF-β1, prestin, and HSP-70 and anti-oxidant activity in the cochlea of noise-exposed rats

Iranian Journal of Basic Medical Sciences
مقاله 5، دوره 23، شماره 5، مرداد 2020، صفحه 594-599    اصل مقاله (542.05 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.41007.9693
نویسندگان
Maryam Bahaloo1؛ Mohammad Ebrahim Rezvani2؛ Ehsan Farashahi Yazd3؛ Fatemeh Zare Mehrjerdi2؛ Mohammad Hossein Davari4؛ Ali Roohbakhsh5؛ Abolfazl Mollasadeghi1؛ Haniyeh Nikkhah3؛ Maryam Vafaei3؛ Amir Houshang Mehrparvar* 1
1Industrial Diseases Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2Department of Physiology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4Department of Occupational Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
5Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Noise-induced hearing loss is one of the most common occupational diseases in industrialized countries and can be affected by various environmental and genetic factors. This study was designed to examine the effect of myricetin in preventing this disorder.
Materials and Methods: Twenty-one Wistar rats were randomly divided into five groups: Non-exposed, noise exposure only, noise exposure with vehicle, noise exposure with myricetin 5 mg/Kg, and noise exposure with myricetin 10 mg/kg. All animals were sacrificed after last noise exposure. The left cochlea was dissected from each rat. It was used for mRNA expression analysis (NOX3, TGF-β1, prestin, and HSP-70). Blood samples were collected to assess superoxide dismutase (SOD) activity, 1, 1 diphenyl picrylhydrazyl (DPPH), and malondialdehyde (MDA) measurements.
Results: Real time-PCR assay revealed that noise decreased NOX3 and increased TGF-β1, prestin, and HSP-70 gene expressions. Administration of myricetin at the dose of 5 mg/kg, but not at 10 mg/kg, significantly reversed these changes. Noise also increased MDA levels and decreased SOD and DPPH scavenging activities. Myricetin at the doses of 5 and 10 mg/kg also reversed these changes.
Conclusion: The findings of this study showed that myricetin at the dose of 5 mg/Kg was able to reverse noise-induced abnormalities in gene expression and oxidant/anti-oxidant balance. It is a possibility that myricetin via enhancement of anti-oxidant activity induced these effects.
کلیدواژه‌ها
Anti-oxidants؛ Gene expression؛ Myricetin؛ NADPH oxidase؛ Noise-induced hearing loss؛ Superoxide dismutase
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