سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Sabet Sarvestani, Fatemeh, Zare, Mohammad Ali, saki, Forough, Koohpeyma, Farhad, al-Abdullah, Ismail H., Azarpira, Negar. (1398). The effect of human wharton’s jelly-derived mesenchymal stem cells on MC4R, NPY, and LEPR gene expression levels in rats with streptozotocin-induced diabetes. , 23(2), 214-223. doi: 10.22038/ijbms.2019.39582.9387
Fatemeh Sabet Sarvestani; Mohammad Ali Zare; Forough saki; Farhad Koohpeyma; Ismail H. al-Abdullah; Negar Azarpira. "The effect of human wharton’s jelly-derived mesenchymal stem cells on MC4R, NPY, and LEPR gene expression levels in rats with streptozotocin-induced diabetes". , 23, 2, 1398, 214-223. doi: 10.22038/ijbms.2019.39582.9387
Sabet Sarvestani, Fatemeh, Zare, Mohammad Ali, saki, Forough, Koohpeyma, Farhad, al-Abdullah, Ismail H., Azarpira, Negar. (1398). 'The effect of human wharton’s jelly-derived mesenchymal stem cells on MC4R, NPY, and LEPR gene expression levels in rats with streptozotocin-induced diabetes', , 23(2), pp. 214-223. doi: 10.22038/ijbms.2019.39582.9387
Sabet Sarvestani, Fatemeh, Zare, Mohammad Ali, saki, Forough, Koohpeyma, Farhad, al-Abdullah, Ismail H., Azarpira, Negar. The effect of human wharton’s jelly-derived mesenchymal stem cells on MC4R, NPY, and LEPR gene expression levels in rats with streptozotocin-induced diabetes. , 1398; 23(2): 214-223. doi: 10.22038/ijbms.2019.39582.9387

The effect of human wharton’s jelly-derived mesenchymal stem cells on MC4R, NPY, and LEPR gene expression levels in rats with streptozotocin-induced diabetes

Iranian Journal of Basic Medical Sciences
مقاله 10، دوره 23، شماره 2، اردیبهشت 2020، صفحه 214-223    اصل مقاله (922.6 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2019.39582.9387
نویسندگان
Fatemeh Sabet Sarvestani1؛ Mohammad Ali Zare1؛ Forough saki2؛ Farhad Koohpeyma2؛ Ismail H. al-Abdullah3؛ Negar Azarpira* 1
1Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, Beckman Research Institute of City of Hope, Duarte, USA
چکیده
Objective(s): Type 1 diabetes (T1D) is an autoimmune disease resulting from inflammatory destruction of islets β-cells. Nowadays, progress in cell therapy, especially mesenchymal stem cells (MSCs) proposes numerous potential remedies for T1D. We aimed to investigate the combination therapeutic effect of these cells with insulin and metformin on neuropeptide Y, melanocortin-4 receptor, and leptin receptor genes expression in TID.
Materials and Methods: One hundreds male rats were randomly divided into seven groups: the control, diabetes, insulin (Ins.), insulin+metformin (Ins.Met.), Wharton’s Jelly-derived MSCs (WJ-MSCs), insulin+metformin+WJ-MSCs (Ins.Met.MSCs), and insulin+WJ-MSCs (Ins.MSCs). Treatment was performed from the first day after diagnosis as diabetes. Groups of the recipient WJ-MSCs were intraportally injected with 2× 10⁶ MSCs/kg at the 7th and 28th days of study. Fasting blood sugar was monitored and tissues and genes analysis were performed.
Results: The blood glucose levels were slightly decreased in all treatment groups within 20th and 45th days compared to the diabetic group. The C-peptide level enhanced in these groups compared to the diabetic group, but this increment in Ins.MSCs group on the 45th days was higher than other groups. The expression level of melanocortin-4 receptor and leptin receptor genes meaningfully up-regulated in the treatment groups, while the expression of neuropeptide Y significantly down-regulated in the treatment group on both times of study.
Conclusion: Our data exhibit that infusion of MSCs and its combination therapy with insulin might ameliorate diabetes signs by changing the amount of leptin and subsequent changes in the expression of neuropeptide Y and melanocortin-4 receptor.
کلیدواژه‌ها
Diabetes mellitus؛ Leptin؛ Melanocortin؛ Neuropeptide Y؛ Receptor؛ Stem cells؛ Wharton jelly
مراجع
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