سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Salehi, Majid, Ai, Arman, Ehterami, Arian, Einabadi, Masoumeh, Taslimi, Alireza, Ai, Armin, Akbarzadeh, Hamta, Ameli, Ghazal Jabal, Farzamfar, Saeed, Shirian, Sadegh, Azimi, Nahal, Sadeghi, Faezeh, Bahrami, Naghmeh, goodarzi, Arash, Ai, Jafar. (1399). In vitro and In vivo Investigation of poly(lactic acid)/hydroxyapatite nanoparticle scaffold containing nandrolone decanoate for the regeneration of critical-sized bone defects. , 7(2), 115-123. doi: 10.22038/nmj.2020.07.004
Majid Salehi; Arman Ai; Arian Ehterami; Masoumeh Einabadi; Alireza Taslimi; Armin Ai; Hamta Akbarzadeh; Ghazal Jabal Ameli; Saeed Farzamfar; Sadegh Shirian; Nahal Azimi; Faezeh Sadeghi; Naghmeh Bahrami; Arash goodarzi; Jafar Ai. "In vitro and In vivo Investigation of poly(lactic acid)/hydroxyapatite nanoparticle scaffold containing nandrolone decanoate for the regeneration of critical-sized bone defects". , 7, 2, 1399, 115-123. doi: 10.22038/nmj.2020.07.004
Salehi, Majid, Ai, Arman, Ehterami, Arian, Einabadi, Masoumeh, Taslimi, Alireza, Ai, Armin, Akbarzadeh, Hamta, Ameli, Ghazal Jabal, Farzamfar, Saeed, Shirian, Sadegh, Azimi, Nahal, Sadeghi, Faezeh, Bahrami, Naghmeh, goodarzi, Arash, Ai, Jafar. (1399). 'In vitro and In vivo Investigation of poly(lactic acid)/hydroxyapatite nanoparticle scaffold containing nandrolone decanoate for the regeneration of critical-sized bone defects', , 7(2), pp. 115-123. doi: 10.22038/nmj.2020.07.004
Salehi, Majid, Ai, Arman, Ehterami, Arian, Einabadi, Masoumeh, Taslimi, Alireza, Ai, Armin, Akbarzadeh, Hamta, Ameli, Ghazal Jabal, Farzamfar, Saeed, Shirian, Sadegh, Azimi, Nahal, Sadeghi, Faezeh, Bahrami, Naghmeh, goodarzi, Arash, Ai, Jafar. In vitro and In vivo Investigation of poly(lactic acid)/hydroxyapatite nanoparticle scaffold containing nandrolone decanoate for the regeneration of critical-sized bone defects. , 1399; 7(2): 115-123. doi: 10.22038/nmj.2020.07.004

In vitro and In vivo Investigation of poly(lactic acid)/hydroxyapatite nanoparticle scaffold containing nandrolone decanoate for the regeneration of critical-sized bone defects

Nanomedicine Journal
مقاله 4، دوره 7، شماره 2، تیر 2020، صفحه 115-123    اصل مقاله (528.87 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/nmj.2020.07.004
نویسندگان
Majid Salehi1، 2؛ Arman Ai3؛ Arian Ehterami4؛ Masoumeh Einabadi5؛ Alireza Taslimi6؛ Armin Ai* 6؛ Hamta Akbarzadeh6؛ Ghazal Jabal Ameli6؛ Saeed Farzamfar7؛ Sadegh Shirian8؛ Nahal Azimi6؛ Faezeh Sadeghi6؛ Naghmeh Bahrami9، 10؛ Arash goodarzi11؛ Jafar Ai7
1Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
2Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
3School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
4Department of Mechanical and Aerospace Engineering, Islamic Azad University, Science and Research Branch, Tehran, Iran
5Department of Biology, Islamic Azad University, Jahrom Branch, Jahrom, Iran
6Dentistry Student, Scientific Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
7Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran 1417755469, Iran
8Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
9Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
10Department of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
11Department of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran
چکیده
Objective(s): Bone tissue engineering is aimed at the fabrication of bone graft to ameliorate bone defects without using autografts or allografts.
Materials and Methods: In the present study, the coprecipitation method was used to prepare hydroxyapatite (HA) nanoparticles containing nandrolone. To do so, 12.5, 25, and 50 mg of nandrolone were loaded into poly(lactic acid) (PLA)/nano-HA, and the freeze casting method was used to fabricate porous scaffolds. The morphology, mechanical strength, wettability, porosity, degradation, blood compatibility, and cellular response of the scaffolds were evaluated using various tests. For further investigation, the developed scaffolds were incorporated into the rat calvaria defect model, and their effects on bone healing were evaluated.
Results: The obtained results indicated that the fabricated scaffolds had the approximate porosity of 80% and compress strength of 6.5 MPa. Moreover, the prepared scaffolds had appropriate hydrophilicity, weight loss, and blood compatibility. Furthermore, the histopathological findings demonstrated that the defects filled with the PLA/nano-HA scaffolds containing 25 mg nandrolone healed better compared to the other study groups.
Conclusion: Therefore, it was concluded that the scaffolds containing nandrolone could be used in bone regeneration.
کلیدواژه‌ها
Bone Healing؛ Freeze Casting Method؛ Hydroxyapatite؛ Nandrolone؛ Scaffold
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