سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Topaloğlu, Ulaş Serkan, Sipahioğlu, Murat Hayri, Güntürk, İnayet, Akgün, Hülya, Doğan, Muhammet Ensar, Sönmez, Gökhan, Elmalı, Ferhan, Yazıcı, Cevat. (1398). Effects of thymoquinone in prevention of experimental contrast-induced nephropathy in rats. , 22(12), 1432-1439. doi: 10.22038/ijbms.2019.13990
Ulaş Serkan Topaloğlu; Murat Hayri Sipahioğlu; İnayet Güntürk; Hülya Akgün; Muhammet Ensar Doğan; Gökhan Sönmez; Ferhan Elmalı; Cevat Yazıcı. "Effects of thymoquinone in prevention of experimental contrast-induced nephropathy in rats". , 22, 12, 1398, 1432-1439. doi: 10.22038/ijbms.2019.13990
Topaloğlu, Ulaş Serkan, Sipahioğlu, Murat Hayri, Güntürk, İnayet, Akgün, Hülya, Doğan, Muhammet Ensar, Sönmez, Gökhan, Elmalı, Ferhan, Yazıcı, Cevat. (1398). 'Effects of thymoquinone in prevention of experimental contrast-induced nephropathy in rats', , 22(12), pp. 1432-1439. doi: 10.22038/ijbms.2019.13990
Topaloğlu, Ulaş Serkan, Sipahioğlu, Murat Hayri, Güntürk, İnayet, Akgün, Hülya, Doğan, Muhammet Ensar, Sönmez, Gökhan, Elmalı, Ferhan, Yazıcı, Cevat. Effects of thymoquinone in prevention of experimental contrast-induced nephropathy in rats. , 1398; 22(12): 1432-1439. doi: 10.22038/ijbms.2019.13990

Effects of thymoquinone in prevention of experimental contrast-induced nephropathy in rats

Iranian Journal of Basic Medical Sciences
مقاله 9، دوره 22، شماره 12، اسفند 2019، صفحه 1432-1439    اصل مقاله (555.47 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2019.13990
نویسندگان
Ulaş Serkan Topaloğlu* 1؛ Murat Hayri Sipahioğlu2؛ İnayet Güntürk3؛ Hülya Akgün4؛ Muhammet Ensar Doğan5؛ Gökhan Sönmez6؛ Ferhan Elmalı7؛ Cevat Yazıcı8
1Department of Internal Medicine, Kayseri City Hospital, Kayseri, Turkey
2Department of Nephrology, Erciyes University Medical Faculty, Kayseri, Turkey
3Department of Medical Biochemistry, Ömer Halisdemir University School of Medicine, Niğde, Turkey
4Department of Pathology, Erciyes University Medical Faculty, Kayseri, Turkey
5Department of Medical Genetics, Erciyes University Medical Faculty, Kayseri, Turkey
6Department of Urology, Kayseri City Hospital, Kayseri, Turkey
7Department of Biostatistics, İzmir Katip Çelebi Üniversitesi Medical Faculty, İzmir, Turkey
8Department of Biochemistry, Erciyes University Medical Faculty, Kayseri, Turkey
چکیده
Objective(s): This study aimed to show the effects of thymoquinone, which is known for its antioxidant, anti-inflammatory, and renal protective effects in contrast-induced nephropathy.
Materials and Methods: This is an experimental study in rats. 7 groups were included within the scope of our study: sham-vehicle (n=3), premedication-control (n=6), model (n=6), isolated thymoquinone (n=3+3), low-dose thymoquinone (n=6), and high-dose thymoquinone (n=7). In addition to 48 hr of water deprivation, we pre-medicated the rats with intra-peritoneal indomethacin and L-NAME administration. After premedication, 12.5 ml/kg dose of a high osmolar contrast agent-diatrizoat (Urografin %76) was administrated. Thymoquinone was administrated in two different doses of 1 mg/kg and 1.75 mg/kg for four days intraperitoneally. Renal functions, histopathological differences, oxidative stress parameters, and inflammatory indicators of rats were evaluated at the end of the study.
Results: Significant decreases were observed in levels of serum creatinine and serum BUN with low-dose thymoquinone (1 mg/kg) administration. In light microscopy, significantly less histopathological damage was observed in the low-dose thymoquinone group compared to the contrast agent group. While high-dose thymoquinone is accepted as ineffective biochemically, toxic evidence was identified histopathologically. There were no significant differences between M and TA groups for serum MDA and SOD levels, which were compared to evaluate oxidative stress (P:0.99, P:0.98; respectively). TNF-α, iNOS, and NF-кB gene expressions were not significantly different between all groups (P:0.748, P:0.531, P:0.910; respectively).
Conclusion: This experimental study has demonstrated for the first time the protective effect of the TQ substance for CIN in 1 mg/kg dose, in the accompaniment of biochemical and histopathological data in rats.
کلیدواژه‌ها
Contrast-induced-nephropathy؛ Inflammation؛ Oxidative stress؛ Nigella sativa؛ Rat؛ Thymoquinone
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