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Iranpak, Forough, Saberzadeh, Jamileh, Vesal, Mahmood, Takhshid, Mohammad Ali. (1398). Sodium valproate ameliorates aluminum-induced oxidative stress and apoptosis of PC12 cells. , 22(11), 1353-1358. doi: 10.22038/ijbms.2019.36930.8804
Forough Iranpak; Jamileh Saberzadeh; Mahmood Vesal; Mohammad Ali Takhshid. "Sodium valproate ameliorates aluminum-induced oxidative stress and apoptosis of PC12 cells". , 22, 11, 1398, 1353-1358. doi: 10.22038/ijbms.2019.36930.8804
Iranpak, Forough, Saberzadeh, Jamileh, Vesal, Mahmood, Takhshid, Mohammad Ali. (1398). 'Sodium valproate ameliorates aluminum-induced oxidative stress and apoptosis of PC12 cells', , 22(11), pp. 1353-1358. doi: 10.22038/ijbms.2019.36930.8804
Iranpak, Forough, Saberzadeh, Jamileh, Vesal, Mahmood, Takhshid, Mohammad Ali. Sodium valproate ameliorates aluminum-induced oxidative stress and apoptosis of PC12 cells. , 1398; 22(11): 1353-1358. doi: 10.22038/ijbms.2019.36930.8804

Sodium valproate ameliorates aluminum-induced oxidative stress and apoptosis of PC12 cells

Iranian Journal of Basic Medical Sciences
مقاله 16، دوره 22، شماره 11، بهمن 2019، صفحه 1353-1358    اصل مقاله (726.02 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2019.36930.8804
نویسندگان
Forough Iranpak1، 2؛ Jamileh Saberzadeh2؛ Mahmood Vesal1؛ Mohammad Ali Takhshid* 2
1Department of Biochemistry, Islamic Azad University of Shiraz, Shiraz, Iran
2Diagnostic Laboratory Sciences and Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Objective(s): According to recent studies, valproate shows some protection against oxidative stress (OS) induced by neurotoxins. Current investigation tried to determine the possible ameliorating effects of sodium valproate (SV) against aluminum (Al)-induced cell death, apoptosis, mitochondrial membrane potential (MMP), and OS in PC12 cells.
Materials and Methods: In this in vitro study, PC12 cells were treated with different concentrations of aluminum maltolate (Almal) with and without SV (50–400 µM).  Cell viability was assessed by MTT assay.  To measure quantitatively the effects of SV on Al-induced apoptosis and reactive oxygen species (ROS), flowcytometry using 7AAD/annexin-V and 2’, 7’-dichlorofluorescein diacetate staining were employed, respectively. MMP was monitored using the retention of rhodamine 123. Catalase (CAT) activity was assayed by the rate of decomposition of hydrogen peroxide.
Results: Exposure of PC12 cells for 48 hr to Almal (125–2000 µM) significantly reduced cell viability (IC50=1090 μM), increased ROS generation and apoptosis, and reduced MMP and CAT activity. SV reduced the Almal-induced cell death and apoptosis. Furthermore, the effects of Almal on ROS generation, catalase activity, and MMP reduction were significantly diminished by SV.  
Conclusion: Data from this study suggest that SV can inhibit Al-induced cell death and apoptosis of PC12 cells via ameliorating OS.
کلیدواژه‌ها
Aluminum maltolate؛ Apoptosis؛ Histone deacetylase inhibitor؛ Oxidative stress؛ Valproic acid
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