سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Gao, Yan, Zhang, Yi, Fan, Yangyang. (1398). Eupafolin ameliorates lipopolysaccharide-induced cardiomyocyte autophagy via PI3K/AKT/mTOR signaling pathway. , 22(11), 1340-1346. doi: 10.22038/ijbms.2019.37748.8977
Yan Gao; Yi Zhang; Yangyang Fan. "Eupafolin ameliorates lipopolysaccharide-induced cardiomyocyte autophagy via PI3K/AKT/mTOR signaling pathway". , 22, 11, 1398, 1340-1346. doi: 10.22038/ijbms.2019.37748.8977
Gao, Yan, Zhang, Yi, Fan, Yangyang. (1398). 'Eupafolin ameliorates lipopolysaccharide-induced cardiomyocyte autophagy via PI3K/AKT/mTOR signaling pathway', , 22(11), pp. 1340-1346. doi: 10.22038/ijbms.2019.37748.8977
Gao, Yan, Zhang, Yi, Fan, Yangyang. Eupafolin ameliorates lipopolysaccharide-induced cardiomyocyte autophagy via PI3K/AKT/mTOR signaling pathway. , 1398; 22(11): 1340-1346. doi: 10.22038/ijbms.2019.37748.8977

Eupafolin ameliorates lipopolysaccharide-induced cardiomyocyte autophagy via PI3K/AKT/mTOR signaling pathway

Iranian Journal of Basic Medical Sciences
مقاله 14، دوره 22، شماره 11، بهمن 2019، صفحه 1340-1346    اصل مقاله (1.03 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2019.37748.8977
نویسندگان
Yan Gao* 1؛ Yi Zhang2؛ Yangyang Fan3
1Function Testing Lab, Shaanxi Provincial People’s Hospital, Xi’an, Shaanxi P.R. China
2ICU Department, Shaanxi Provincial People’s Hospital. Xi’an, Shaanxi P.R. China
3Obstetrical Department, Shaanxi Provincial People’s Hospital. Xi’an, Shaanxi P.R. China
چکیده
Objective(s): Eupafolin, a major active component of Eupatorium perfoliatum L., has anti-inflammatory and anti-oxidant properties. Lipopolysaccharide (LPS) is responsible for myocardial depression. A line of evidences revealed that LPS induces autophagy in cardiomyocytes injury. This study aims to evaluate the effects of eupafolin on LPS-induced cardiomyocyte autophagy.
Materials and Methods: The effect of LPS on cell viability was examined by CCK-8. Autophagic protein 2 light chain 3 (LC3II), which was regulated by LPS and eupafolin, was examined using immunofluorescent staining. The expression levels of Beclin-1 and p62 were detected by western blotting. The effects of eupafolin on phosphatidylinositol-3-kinase/ protein kinase B/ mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway were also evaluated by western blotting and immunofluorescent staining.
Results: Eupafolin pretreatment reduced the expression of LC3II and Beclin-1, whereas p62 was significant increased. In addition, eupafolin promoted expression of PI3K/AKT/mTOR signaling pathway and mTOR inhibitor rapamycin reversed the inhibitory effects on LPS-induced cardiomyocyte autophagy.
Conclusion: Eupafolin exerts anti-autophagy activity via activation of PI3K/AKT/mTOR signaling pathway.
کلیدواژه‌ها
Autophagy؛ Cardiomyocyte؛ Eupafolin؛ Lipopolysaccharides؛ Mammalian target of rapamycin
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