Neuroprotective effects of Tiliacora triandra leaf extract in a mice model of cerebral ischemia reperfusion | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 11، دوره 10، شماره 2، خرداد و تیر 2020، صفحه 202-212 اصل مقاله (766.33 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2019.13877 | ||
| نویسندگان | ||
| Wachiryah Thong-asa* ؛ Vasakorn Bullangpoti | ||
| Animal Toxicology and Physiology Specialty Research Unit (ATPSRU), Physiology Division, Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand. | ||
| چکیده | ||
| Objective: The present study investigated possible neuroprotective effects of ethanolic extract of Tiliacora triandra leaf against cerebral ischemic-reperfusion injury in mice. Materials and Methods: Forty male Institute of Cancer Research (ICR) mice were randomly divided into five groups: (1) Sham + 10% Tween 80, (2) bilateral common carotid artery occlusion (BCCAO) + 10% Tween 80, (3) BCCAO + T. triandra 300 mg/kg, (4) BCCAO + T. triandra 600 mg/kg and (5) BCCAO + quercetin 10 mg/kg. Cerebral ischemic-reperfusion (IR) was induced by 30 min of BCCAO followed by 45 min of reperfusion. After IR induction, total brain protein, calcium, malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), and reduced glutathione (GSH), as well as brain infraction and histopathological changes in vulnerable brain areas, such as the cerebral cortex and hippocampus, were evaluated. Results: The results showed that 2 weeks of pretreatment with T. triandra leaf extract at doses of 300 and 600 mg/kg significantly reduced calcium and MDA, but increased GSH and SOD and CAT activities. The extract significantly attenuated brain infarction and neuronal death in the cerebral cortex and hippocampus. Conclusion: We demonstrated the neuroprotective effects of T. triandra leaf extract against cerebral IR injury in mice. | ||
| کلیدواژهها | ||
| Tiliacora triandra؛ Ischemic-reperfusion injury؛ Bilateral common carotid artery occlusion؛ Cerebral cortex؛ Hippocampus؛ Lipid peroxidation | ||
| مراجع | ||
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