Pomegranate peel extract inhibits internalization and replication of the influenza virus: An in vitro study | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 5، دوره 10، شماره 2، خرداد و تیر 2020، صفحه 143-151 اصل مقاله (359.45 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2019.13855 | ||
| نویسندگان | ||
| Mohammad-Taghi Moradi1؛ Ali Karimi* 2؛ Mahmoud Rafieian-kopaei1؛ Mohammad Rabiei-Faradonbeh3؛ Hassan Momtaz4 | ||
| 1Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran. | ||
| 2Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran. | ||
| 3Department of Pathobiology, Infectious Disease and Public Health, School of Animal and Veterinary Sciences, University of Adelaide, Adelaide, Australia. | ||
| 4Department of Microbiology, College of Veterinary Medicine, Shahrekord Branch, Islamic Azad University. | ||
| چکیده | ||
| Objective: Influenza virus, which is associated with high level of morbidity and mortality, has been recently considered a public health concern; however, the methods of choice to control and treat it are limited. Our previous study showed anti-influenza virus activity of pomegranate peel extract (PPE). In this study, the mechanism through which PPE acts against influenza virus A/Puerto Rico/8/34 (H1N1; PR8) was investigated. Materials and Methods:Ethyl alcohol extract of pomegranate (Punica granatum L.) peel was prepared, and the action mechanism of PPE in inhibiting influenza replication was studied by time-of-drug-addition assay, virucidal activity, RNA replication, hemagglutination inhibition assay, viral mRNA expression, and western blot analysis. Results: PPE inhibited viral polymerase activity, viral RNA replication, and viral protein expression but could not affect hemagglutination inhibition and virucidal activity. According to time-of-drug-addition assay results, PPE inhibited the virus adsorption and early steps of influenza replication. Conclusion: This study demonstrated that the antiviral effect of PPE on influenza virus is most probably associated with inhibition of viral adsorption and viral RNA transcription. | ||
| کلیدواژهها | ||
| Anti-influenza virus؛ Pomegranate؛ Punica granatum L؛ Mechanisms | ||
| مراجع | ||
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