Conditioned medium obtained from human amniotic membrane-derived mesenchymal stem cell attenuates heart failure injury in rats | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 2، دوره 22، شماره 11، بهمن 2019، صفحه 1253-1258 اصل مقاله (945.75 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2019.36617.8722 | ||
| نویسندگان | ||
| Solmaz Nasseri Maleki1؛ Nahid Aboutaleb* 1؛ Donya Nazarinia2؛ Sara Allahverdi Beik1؛ Asadollah Qolamian1؛ Maliheh Nobakht3 | ||
| 1Physiology Research Center and Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 2Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 3Department of Histology and Neuroscience, Anti-microbial Resistance Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Objective(s): Heart failure (HF) is one of the leading causes of death worldwide. Due to beneficial effects of stem cells, paracrine secretion of them has recently been used by researchers. The purpose of this study was to investigate the effects of intravenous injection (IV) of conditioned medium (CM) of human amniotic membrane-derived mesenchymal stem cell (MSC-CM) on HF. Materials and Methods: Male Wistar rats (n=35, 180 g) were randomly divided into five groups: sham, HF, HF+MSC-CM, HF+culture medium and HF+phosphate-buffered saline (PBS). To induce HF, isoproterenol (170 mg/kg/d) was injected subcutaneously for 4 consecutive days. After 28 days, induction of HF was evaluated by echocardiography. A day after echocardiography, 50 μg culture medium/5 ml PBS in HF+culture medium group, 50 μg MSC-CM/5 ml PBS in HF+MSC-CM group and 5 ml PBS in HF+PBS group were injected two times for 4 successive days. The echocardiography was performed 4 weeks after the last injection of isoproterenol. To evaluate the fibrosis, morphology, and cardiac function, Trichrome Masson’s staining, Hematoxylin and Eosin staining and echocardiography were performed, respectively. Results: CM significantly increased fractional shortening and ejection fraction, and also significantly decreased apoptotic nuclear condensation. Moreover, significant decreased level of fibrosis and increased level of angiogenesis was observed in the treatment group (P<0.05). Conclusion: Our results indicated that IV injection of CM has therapeutic effects on HF by reducing fibrosis and preventing the progression of failure due to its paracrine effects. | ||
| کلیدواژهها | ||
| Adipose tissue-derived-mesenchymal stem cells؛ Apoptosis؛ Conditioned medium؛ Fibrosis؛ Heart failure | ||
| مراجع | ||
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