Mohammadzadeh, Fatemeh, Alihemmati, Alireza, Pirpour Tazehkand, Abbas, Darabi, Masoud, Mehdizadeh, Amir. (1398). Early oleate deficiency leads to severe defects in fetal rat liver development. , 22(9), 1010-1015. doi: 10.22038/ijbms.2019.35084.8345
Fatemeh Mohammadzadeh; Alireza Alihemmati; Abbas Pirpour Tazehkand; Masoud Darabi; Amir Mehdizadeh. "Early oleate deficiency leads to severe defects in fetal rat liver development". , 22, 9, 1398, 1010-1015. doi: 10.22038/ijbms.2019.35084.8345
Mohammadzadeh, Fatemeh, Alihemmati, Alireza, Pirpour Tazehkand, Abbas, Darabi, Masoud, Mehdizadeh, Amir. (1398). 'Early oleate deficiency leads to severe defects in fetal rat liver development', , 22(9), pp. 1010-1015. doi: 10.22038/ijbms.2019.35084.8345
Mohammadzadeh, Fatemeh, Alihemmati, Alireza, Pirpour Tazehkand, Abbas, Darabi, Masoud, Mehdizadeh, Amir. Early oleate deficiency leads to severe defects in fetal rat liver development. , 1398; 22(9): 1010-1015. doi: 10.22038/ijbms.2019.35084.8345

Early oleate deficiency leads to severe defects in fetal rat liver development

Iranian Journal of Basic Medical Sciences
مقاله 6، دوره 22، شماره 9، آذر 2019، صفحه 1010-1015    اصل مقاله (582.97 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2019.35084.8345
نویسندگان
Fatemeh Mohammadzadeh1؛ Alireza Alihemmati2؛ Abbas Pirpour Tazehkand3؛ Masoud Darabi* 1، 3؛ Amir Mehdizadeh4، 5
1Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
4Endocrine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5Comprehensive Health Lab, Tabriz University of Medical Sciences, Tabriz, Iran
چکیده
Objective(s): Oleate can be produced through de novo synthesis, which contributes to biological processes and signaling pathways. However, the role of this non-essential fatty acid in hepatic development remains unclear. The current study aimed to evaluate the influence of early oleate deficiency induced by the inhibitor of de novo oleate synthesis MF-438 on fetal rat liver development.
Materials and Methods: Female Wistar rats with an average weight of 200±20 g were subjected to this study. After mating, pregnant rats were divided into three groups and gavaged with the vehicle, MF 438 or MF-438 plus oleate from day 3 of pregnancy for five days. Obtained fetuses were sacrificed and the liver tissues were retrieved. Hepatic morphological index, biochemical markers, and gene expression of hepatic development markers were analyzed using Hematoxylin-Eosine, spectrometry, and real-time PCR techniques, respectively.
Results: Relatively, deficient morphological indices and hepatic maturation markers were observed in fetus livers of the inhibitor-treated group. In comparison to the other two groups, total hepatic protein and glycogen content were increased with treatment of MF-438 plus oleate. Hepatocyte nuclear factor 1α, alpha fetoprotein, albumin, and cytochrome P450 gene expression were also significantly increased in the group treated with both MF-438 and oleate.
Conclusion: Our data indicate that oleate availability during early embryo development is linked with fetal rat liver development.
کلیدواژه‌ها
Development؛ Embryo؛ Hepatocytes؛ Monounsaturated fatty acids؛ Pregnancy
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