Taheri Moghadam, Mahin, Asadi Fard, Yousef, saki, Ghasem, Nikbakht, Roshan. (1398). Effect of vitamin D on apoptotic marker, reactive oxygen species and human sperm parameters during the process of cryopreservation. , 22(9), 1036-1043. doi: 10.22038/ijbms.2019.36258.8634
Mahin Taheri Moghadam; Yousef Asadi Fard; Ghasem saki; Roshan Nikbakht. "Effect of vitamin D on apoptotic marker, reactive oxygen species and human sperm parameters during the process of cryopreservation". , 22, 9, 1398, 1036-1043. doi: 10.22038/ijbms.2019.36258.8634
Taheri Moghadam, Mahin, Asadi Fard, Yousef, saki, Ghasem, Nikbakht, Roshan. (1398). 'Effect of vitamin D on apoptotic marker, reactive oxygen species and human sperm parameters during the process of cryopreservation', , 22(9), pp. 1036-1043. doi: 10.22038/ijbms.2019.36258.8634
Taheri Moghadam, Mahin, Asadi Fard, Yousef, saki, Ghasem, Nikbakht, Roshan. Effect of vitamin D on apoptotic marker, reactive oxygen species and human sperm parameters during the process of cryopreservation. , 1398; 22(9): 1036-1043. doi: 10.22038/ijbms.2019.36258.8634

Effect of vitamin D on apoptotic marker, reactive oxygen species and human sperm parameters during the process of cryopreservation

Iranian Journal of Basic Medical Sciences
مقاله 9، دوره 22، شماره 9، آذر 2019، صفحه 1036-1043    اصل مقاله (655.01 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2019.36258.8634
نویسندگان
Mahin Taheri Moghadam* 1؛ Yousef Asadi Fard2؛ Ghasem saki1؛ Roshan Nikbakht3
1Cellular and Molecular Research center, Ahvaz Jundishapur University of Medical Sciences
2Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3Fertility, Infertility and Perinatology center, Imam Khomeini Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
چکیده
Objective(s): Sperm cryopreservation plays an undeniable role in assisted reproductive technology. However, this process significantly reduces the motility, viability, morphology and nuclear integrity of sperm. Reasons of these changes were oxidative stress and apoptosis. The aim of this study was to evaluate the influence of vitamin D on the survival and integrity of fertile sperm after cryopreservation.
Materials and Methods: Semen sample of 18 males with normal parameters was used. After swimming up, each sample was divided into two parts. 20 µmol vitamin D was added to one part as experimental group and the other part was left untreated as control group. The samples in all groups were frozen for 14 days. Post-thawing, the groups were evaluated for sperm motility, and viability using eosin staining, morphology using the Diff-Quick staining and apoptosis by TUNEL, Annexin-V and caspase-3 activity assay. By using nitrobluetetraxolium test and thiobarbituric acid, the reactive oxygen species (ROS) and lipid peroxidation of sperms were measured, respectively.
Results: In comparison with control groups, motile and viable sperm concentration was substantially higher in treated groups (P-value<0.05); however, morphological analysis did not show any remarkable changes. Also, ROS and lipid peroxidation values were dramatically reduced by vitamin D (P-value<0.05). TUNEL and Annexin assay for apoptosis were considerably lower in treated groups (P-value<0.05), but caspase activity assay revealed no significant difference between groups.
Conclusion: The results have shown that the addition of vitamin D to a freezing medium leads to higher quality and function of human sperm.
کلیدواژه‌ها
Apoptosis؛ Human sperm freezing؛ Infertility؛ Oxidative stress؛ Vitamin D
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