Cytotoxic activity of caffeic acid and gallic acid against MCF-7 human breast cancer cells: An in silico and in vitro study | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 9، دوره 9، شماره 6، بهمن و اسفند 2019، صفحه 574-586 اصل مقاله (852.94 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2019.13475 | ||
| نویسندگان | ||
| Hasan Rezaei-Seresht1؛ Hamid Cheshomi* 2، 3؛ Farahnaz Falanji4؛ Fatemeh Movahedi-Motlagh2، 5؛ Maryam Hashemian6، 7؛ Erfan Mireskandari8 | ||
| 1Traditional and Complementary Medicine Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran | ||
| 2Cellular and Molecular Research Center, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran. | ||
| 3Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 4Cellular and Molecular Research Center, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran | ||
| 5Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran | ||
| 6Department of Nutrition, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran. | ||
| 7Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran | ||
| 8Department of Chemistry, School of Sciences, Hakim Sabzevari University, Sabzevar, Iran | ||
| چکیده | ||
| Objective: Phenolic compounds have been considered inhibitors of various cancers. Material and Methods: In this study, caffeic acid and gallic acid were appraised for their possible effects on apoptotic genes expression in a breast cancer cell line in vitro. We also evaluated ligand interaction and ligand binding with estrogen receptor alpha by molecular docking. To determine half maximal inhibitory concentration, MCF-7 cells were treated with different concentrations of caffeic acid and gallic acid by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Furthermore, morphological changes in cells and alterations in P53, Mcl-1 and P21 gene expression were studied by real-time RT-PCR. Also, protein network and different interactions between the desired genes were analyzed using GeneMANIA database. Results: Evaluation of cell survival by MTT assay revealed that the half-maximal inhibitory concentration values for caffeic acid and gallic acid against MCF-7 cells, were 159 and 18 µg/ml, respectively. These compounds were found to affect P53, Mcl-1 and P21 gene expression; this alteration in gene expression probably occurred along with the activation of intrinsic apoptotic signaling pathway. Conclusion: Via apoptosis induction, caffeic acid and gallic acid have induce toxic effects and morphological changes in breast cancer cells, suggesting their possible future application as antitumor agents. | ||
| کلیدواژهها | ||
| Caffeic acid؛ Gallic acid؛ MCF-7؛ P53؛ P21؛ Mcl-1 | ||
| مراجع | ||
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