سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Mehravaran, Ahmad, Mirahmadi, Hadi, Akhtari, Javad. (1398). Liposomes containing the imiquimod adjuvant as a vaccine in the cutaneous leishmaniasis model. , 7(1), 29-39. doi: 10.22038/nmj.2020.07.04
Ahmad Mehravaran; Hadi Mirahmadi; Javad Akhtari. "Liposomes containing the imiquimod adjuvant as a vaccine in the cutaneous leishmaniasis model". , 7, 1, 1398, 29-39. doi: 10.22038/nmj.2020.07.04
Mehravaran, Ahmad, Mirahmadi, Hadi, Akhtari, Javad. (1398). 'Liposomes containing the imiquimod adjuvant as a vaccine in the cutaneous leishmaniasis model', , 7(1), pp. 29-39. doi: 10.22038/nmj.2020.07.04
Mehravaran, Ahmad, Mirahmadi, Hadi, Akhtari, Javad. Liposomes containing the imiquimod adjuvant as a vaccine in the cutaneous leishmaniasis model. , 1398; 7(1): 29-39. doi: 10.22038/nmj.2020.07.04

Liposomes containing the imiquimod adjuvant as a vaccine in the cutaneous leishmaniasis model

Nanomedicine Journal
مقاله 4، دوره 7، شماره 1، فروردین 2020، صفحه 29-39    اصل مقاله (693.8 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/nmj.2020.07.04
نویسندگان
Ahmad Mehravaran* 1، 2؛ Hadi Mirahmadi1، 2؛ Javad Akhtari3
1Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis institute, Zahedan University of Medical Sciences, Zahedan, Iran
2Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
3Toxoplasmosis Research Center, Department of Medical Nanotechnology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
چکیده
Objective(s): Attempts to produce vaccines for leishmaniasis need adjuvants to trigger the kind of immune reaction required for protection. In this study, we examined the properties of the TLR7 agonist imiquimod, a vaccine adjuvant, making use of a live model of infection where the immune reactions could be identified prior to and following the challenge of infection.
Materials and Methods: The liposomes of EPC containing the imiquimod adjuvant were prepared and characterized for protein concentration, surface charge, and particle size. Vaccination was done using the soluble Leishmania antigen (SLA) as a first-generation vaccine model in the liposomal state to vaccinate BALB/c mice against the challenge of leishmania major. BALB/c mice were vaccinated subcutaneously, three times at a two-week interval. Parasite burden, footpad swelling, IgG isotype, as well as the level of IL-4 and IFN-γ were assessed as the protection criteria.
Results: The group of mice vaccinated by Lip+Imiquimod+SLA demonstrated a lower amount of footpad swelling and parasite burden than the buffer group. In addition, the highest level of IFN-γ and the lowest level of IL-4 production was noticed in the splenocytes of the mice vaccinated with the formulation of Lip+Imiquimod+SLA.
Conclusion: These results imply that imiquimod added to the formulation of liposomes is able to modulate the immune reaction of the BALB/c mice vaccinated preferably to a Th1 reaction rather than a Th2 reaction which can also lead to partial protection against the challenge of Leishmania.
کلیدواژه‌ها
Adjuant؛ Leishmaniasis؛ Imiquimod؛ Liposome؛ Vaccine
مراجع

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