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Bahr, Hoda I, Hamad, Rania, Ismail, Shimaa A A. (1398). The impact of Lactobacillus acidophilus on hepatic and colonic fibrosis induced by ethephon in a rat model. , 22(8), 956-962. doi: 10.22038/ijbms.2019.32936.7866
Hoda I Bahr; Rania Hamad; Shimaa A A Ismail. "The impact of Lactobacillus acidophilus on hepatic and colonic fibrosis induced by ethephon in a rat model". , 22, 8, 1398, 956-962. doi: 10.22038/ijbms.2019.32936.7866
Bahr, Hoda I, Hamad, Rania, Ismail, Shimaa A A. (1398). 'The impact of Lactobacillus acidophilus on hepatic and colonic fibrosis induced by ethephon in a rat model', , 22(8), pp. 956-962. doi: 10.22038/ijbms.2019.32936.7866
Bahr, Hoda I, Hamad, Rania, Ismail, Shimaa A A. The impact of Lactobacillus acidophilus on hepatic and colonic fibrosis induced by ethephon in a rat model. , 1398; 22(8): 956-962. doi: 10.22038/ijbms.2019.32936.7866

The impact of Lactobacillus acidophilus on hepatic and colonic fibrosis induced by ethephon in a rat model

Iranian Journal of Basic Medical Sciences
مقاله 16، دوره 22، شماره 8، آبان 2019، صفحه 956-962    اصل مقاله (815 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2019.32936.7866
نویسندگان
Hoda I Bahr* 1؛ Rania Hamad2؛ Shimaa A A Ismail3
1Biochemistry Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt
2Pathology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt
3Clinical Pathology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt
چکیده
Objective(s): The study is aimed to elucidate the impact of antioxidant, anti-inflammatory and antifibrosis properties of Lactobacillus acidophilus (L. acidophilus) on liver and colon in ethephon treated rats through measuring Pro- inflammatory cytokines, oxidative stress index, lysosomal cathepsin-D enzyme activity and fibrosis markers.
Materials and Methods: Rats divided into three groups; Group 1: distilled water control, Group 2: rats at day 16 from experiment beginning were orally received ethephon 50 mg/ kg BW in distilled water once daily for 60 days.  Group 3: rats were orally received L. acidophilus enriched diet 1% (w/w) for 15 days as prophylactic, then received both L. acidophilus enriched diet 1% (w/w) and ethephon 50 mg/kg BW for 60 days.
Results: Ethephon exerts hepatic and colonic oxidative stress, inflammatory response and fibrosis through NF-κB activation. On contrary, L. acidophilus supplementation evokes hepatoprotective properties as revealed by decreased serum AST, ALT, γ -GT and increased IGF-1. L. acidophilus exerts antioxidant and anti-inflammatory properties as indicated by decreased TOS, OSI, TNF-α, IL-1β, cathepsins D activity, NF-κB expression and increased TAC, lysosomal membrane stability. L. acidophilus shows antifibrotic activity as demonstrated by down-regulation of TGF-β1, α-SMA, collagen expression.
Conclusion: L. acidophilus possess antioxidant, anti -inflammatory and antifibrotic activity through inhibition of NF-kB.
کلیدواژه‌ها
Ethephon؛ Immunity؛ L. acidophilus؛ NF-κB؛ α-SMA
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